小中大楼主的实验有悖于伦理吧
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Fertility preservation for young patients with cancer: who isat risk and what can be offered?
Lancet Oncol 2005; 6: 209–18
W Hamish B Wallace, Richard A Anderson, D Stewart Irvine
Estimates suggest that by 2010, one in 715 people in the UK will have survived cancer during childhood. Withincreasing numbers of children cured, attention has focused on their quality of life. We discuss the causes of impairedfertility after cancer treatment in young people, and outline which patients are at risk and how their gonadal functionshould be assessed. With the report of a livebirth after orthotopic transplantation of cryopreserved ovarian tissue andthe continued development of intracytoplasmic sperm injection for men with poor sperm quality, we assess established and experimental strategies to protect or restore fertility, and discuss the ethical and legal issues that arise.
1. 为什么运输卵巢组织时用冰块，而不是37C？
有文献是这样做的，Tissue was immediately transported from the operat-ing room to the research laboratory in HEPES-buffered Modified Eagle Medium (HEPES-MEM; Gibco) on ice.
Ovarian cortical fragments were obtained using biopsy grasping forceps from an avascular portion of the ovary devoid of visible follicles or luteal tissue, and before elec-
trocoagulation of the puncture site. The specimens wereimmediately transported to the laboratory in Leibovitz L-15 medium (Eurobio, Les Ulis, France) at 4°C. Ovarian cortex was dissected and cut into small pieces (1 mm in thicknessand 0.5 to 1 cm2 in area) on ice.
我自己是觉得刚从手术室取得的组织如果在冰上可以减少组织缺血，有助于组织的存活，有助于卵泡的存活。不知道您说的37度有什么理由，我也看到过37度的，那是从人卵巢组织中机械分离GV期的卵，然后做IVM。
