小中大[分享帖]细胞电子显微镜《CELLULAR ELECTRON MICROSCOPY》
2007年的书，侧重新兴技术
书中很多精彩图片
CELLULAR ELECTRON MICROSCOPY
ISBN: 9780123706478
Edited By
J. McIntosh
Description
Recent advances in the imaging technique electron microscopy (EM) have improved the method, making it more reliable and rewarding, particularly in its description of three-dimensional detail. This book will help biologists from many disciplines understand modern EM and the value it might bring to their own work. The book?s five sections deal with all major issues in EM of cells: specimen preparation, imaging in 3-D, imaging and understanding frozen-hydrated samples, labeling macromolecules, and analyzing EM data. Each chapter was written by scientists who are among the best in their field, and some chapters provide multiple points of view on the issues they discuss. Each section of the book is preceded by an introduction, which should help newcomers understand the subject. The book shows why many biologists believe that modern EM will forge the link between light microscopy of live cells and atomic resolution studies of isolated macromolecules, helping us toward the goal of an atomic resolution understanding of living systems.
全书分4部分
1.Preparing Cells for Electron Microscopy
2.Imaging Fixed Cells in Three Dimensions
3.Imaging Frozen‐Hydrated Cells and Cell Parts
4.Localizing Macromolecules in Cells
5.Aspects of Data Collection and Analysis
Introduction: McIntosh - A new era in electron microscopy of cells
Preparative Methods for Cellular Electron Microscopy
1. The physics of rapid cooling and its implications for cryo-immobilization of cells
2. Cryopreparation Methods for Electron Microscopy of Selected Model Systems
3. Cryo-preparation methodology for plant cell biology
4. Correlative Light and Electron Microscopy of Early C. elegans Embryos in Mitosis
Imaging Fixed Cells in Three Dimensions
5. Understanding microtubule organizing centers by comparing mutant and wild-type structures with electron tomography
6. Whole Cell Investigation of Microtubule Cytoskeleton Architecture by Electron Tomography
7. Electron Microscopy of archaea
8. Reconstructing Mammalian Membrane Architecture by Large Area Cellular Tomography
9. Visualization of Membrane-Cytoskeletal Interactions during Plant Cytokinesis
10. Electron Tomographic Methods for Studying the Chemical Synapse
11. Using electron microscopy to understand functional mechanisms of chromosome alignment on the mitotic spindle
12. EM Analysis of the Leading Edge in Migrating Cells
13. Imaging actomyosin in situ
