小中大这是其他群友关于Hela的经验：
1、
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2、
关于HELA 细胞的培养（引自peakw战友，见下帖
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HELA 细胞生长很快一般一天既可传代。用含有10%小牛血清的DMEM培养。在传代时，将培基吸掉。然后用PBS洗一下，再加0.05%的胰酶放在培养箱中消化30秒，镜下观察细胞变圆即可用有10%小牛血清的DMEM终止消化，吸管吹打细胞后加入适量含10%小牛血清的DMEM放入CO2培养箱培养。
3、
可与自己的操作对比一下看有何不同，另附上ATCC关于Hela细胞传代的Protocol:
Subculturing: Protocol:
1）Remove and discard culture medium.
2）Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
3）Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
4）Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
5）Add appropriate aliquots of the cell suspension to new culture vessels.
6）Incubate cultures at 37°C.
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