小中大你可以search一下，这个问题已经很多次被讨论过了，ecv-304现在已经不被认为是血管内皮细胞了，atcc已经不买这种细胞了。
Lewiszhou 曾发贴：
Recently, my classmates E-mail me about ECV-304 Cell.
The cell in the cell line ECV-304 are not endothelial cell, but are derivated from the tumor cells of a patient with bladder carcinoma.
Some papers about it:
1．Dirks WG, Macleod RAF, Drexler HG. ECV-304(ENDOTHELIAL)is really T24(bladder carcinoma):cell line cross-contamination at source. In Vitro Cell Dev Biol 35:558~559 1999;
2. Drexler HG, Quentmerier H,Driks WG et al. Bladder carcinoma cell line ECV-304 is not a model system for endothelial cells.In Vitro Cell Dev Biol 38:185-186,2002;
3. Brown J, Reading SJ, Jones S et al. Ciritical evaluation of ECV-304 as a human and functional responses:A comparison with the human bladder cancer derived epithelial line T24/83. Lab Invest 2000;80:37-45
4. Tanabe H, Takada Y, Minegishi D, et al. All line individualization by SIR multiplex system in the cell bank found cross-contamination between ECV-304 and EJ-T24. Tiss cult Res Commun 1999;18:329-338;
If you want to get more informatin about it ,please visit the following website:
cuturl('http://www.dsmz.de/mutz/mutz310.htm')
cuturl('http://www.ecacc.org.uk')
using key word: ECV ,then you will get some informations.
Lewis_1121 edited on 2003-09-09 14:10
还有很多，你可以查一下，建议你如果做血管内皮细胞的实验的话，最好不要用ecv-304。
