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标题:【求助】人胰腺癌细胞株

toy[使用道具]
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发表于 2012-9-12 12:35 资料 个人空间 短消息  加为好友 

 小中大

【求助】人胰腺癌细胞株

急求胰腺癌细胞株!

除SW1990、BxPC3、PaTu8988以外。

请提供裸鼠注射后的成瘤时间。

经过朋友们的多方帮助，已经与两处细胞库取得联系，准备购买中，谢谢大家！
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DDD[使用道具]
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发表于 2012-9-12 12:36 资料 个人空间 短消息  加为好友 

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只有人胰腺癌细胞pc-2，没有成瘤时间，也没文章 ！
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orangecake[使用道具]
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发表于 2012-9-12 12:37 资料 个人空间 短消息  加为好友 

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只有人胰腺癌细胞pc-2，没有成瘤时间，也没文章 ！

———————————————————————————————

该胰腺癌细胞株有无正规的来源与出处？

此细胞株有无制备裸鼠肿瘤模型的经历？

还有些具体问题请收邮件。
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BUK[使用道具]
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发表于 2012-9-12 12:37 资料 个人空间 短消息  加为好友 

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Perhaps,I recommend that you should ask ATCC for your胰腺癌细胞株 of SCI.
If you need product information for your items then you can contact Tech Department via email at tech@atcc.org.

Sorry,I also only know the following information at present .

HuP-T3 human pancreas adenocarcinoma, ascitic fluid 人的胰腺癌细胞 HTL96006
HuP-T4 human pancreas adenocarcinoma, ascitic fluid 人的胰腺癌细胞 HTL95006
SFPAC-1   胰腺癌细胞
AR42J   大鼠成纤维细胞  胰腺癌  n.d.
ANC-1 人胰腺癌细胞
PANC-1 人胰腺癌细胞
PSN1 human pancreas adenocarcinoma 人的胰腺癌细胞 HTL97008
CFPAC-1 human pancreas adenocarcinoma, metastatic 人的胰腺癌细胞 HTL01019
SFPAC-1  人胰腺癌细胞
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发表于 2012-9-12 12:38 资料 个人空间 短消息  加为好友 

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Cell Lines

ATCC Number: CRL-1469 Price: $185.00

Designation: PANC-1 Depositors: M Lieber

Biosafety Level: 1 Shipped: frozen

Medium &
Serum: See Propagation Growth
Properties: adherent

Organism: Homo sapiens (human) Morphology: epithelial


Source: Organ: pancreas
Tissue: duct
Disease: epithelioid carcinoma

Permits/Forms:

In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

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发表于 2012-9-12 12:39 资料 个人空间 短消息  加为好友 

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DNA Profile
(STR): Amelogenin: X
CSF1PO: 10,12
D13S317: 11
D16S539: 11
D5S818: 11,13
D7S820: 8,10
THO1: 7,8
TPOX: 8,11
vWA: 15
Cytogenetic Analysis: Chromosome studies indicate a modal number of 63 with 3 distinct marker chromosomes and a small ring chromosome. This is a hypertriploid human cell line. The modal chromosome number was 61, occurring in 32% of cells.
However, cells with 63 chromosomes also occurred at a high frequency (22%). The rate of cells with higher ploidies was 8.5%.
Isoenzymes: G6PD, B
Age: 56 years
Gender: male
Ethnicity: Caucasian
Comments: Growth is inhibited by 1 unit/ml L-asparaginase.
The cells will grow in soft agar.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 90%; fetal bovine serum, 10%

Temperature: 37.0 C
Subculturing: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Doubling Time: 52 hrs
Related
Products: Recommended medium (without the additional supplements or serum described under ATCC Medium) - ATCC 30-2002
recommended serum - ATCC 30-2020
References: 22850: Lieber M , et al. Establishment of a continuous tumor-cell line (panc-1) from a human carcinoma of the exocrine pancreas. Int. J. Cancer 15: 741-747, 1975. PubMed: 1140870
22859: Wu MC , et al. Mechanism of sensitivity of cultured pancreatic carcinoma to asparaginase. Int. J. Cancer 22: 728-733, 1978. PubMed: 363626
23079: Lan MS , et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 2334903
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发表于 2012-9-12 12:40 资料 个人空间 短消息  加为好友 

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Cell Lines

ATCC Number: CRL-1492 Price: $185.00

Designation: AR42J Depositors: NW Jessop

Biosafety Level: 1 Shipped: frozen

Medium &
Serum: See Propagation Growth
Properties: adherent

Organism: Rattus norvegicus (rat) deposited as Rattus sp. Morphology: epithelial


Source: Organ: pancreas
Tissue: exocrine
Disease: tumor


Cellular
Products: amylase and other exocrine enzymes [22185]
Permits/Forms:

In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

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Receptors: insulin, expressed
glucocorticoid, expressed
Tumorigenic: Yes, in athymic mice
Strain: Wistar
Comments: Secretory activity is inducible by glucocorticoid stimulation, and is accompanied by extensive re-organization of the endoplasmic reticulum.
Propagation: ATCC complete growth medium: Ham's F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 80%; fetal bovine serum, 20%
Growth Conditions: The cells cells grow slowly, in clusters. They tend to pile up and appear refractile.
Temperature: 37.0 C Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing: Protocol: Monolayer never becomes confluent. Subculture when patches of cells start forming "domes".
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37癈 to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximately 125 xg for 5 to 10 minutes. Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.
Incubate cultures at 37癈.
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Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Every 3 to 4 days. May need to only add media initially, do not fluid change until cells attach well.
Preservation: Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related
Products: Recommended medium (without the additional supplements or serum described under ATCC Medium) - ATCC 30-2004
recommended serum - ATCC 30-2020
References: 22185: Jessop NW , Hay RJ . Characteristics of two rat pancreatic exocrine cell lines derived from transplantable tumors. In Vitro 16: 212, 1980.
22384: Longnecker DS , et al. Transplantation of azaserine-induced carcinomas of pancreas in rats. Cancer Lett. 7: 197-202, 1979. PubMed: 509403
22884: Cockell M , et al. Identification of a cell-specific DNA-binding activity that interacts with a transcriptional activator of genes expressed in the acinar pancreas. Mol. Cell. Biol. 9: 2464-2476, 1989. PubMed: 2788241
22978: Roux E , et al. The cell-specific transcription factor PTF1 contains two different subunits that interact with the DNA. Genes Dev. 3: 1613-1624, 1989. PubMed: 2612907
23083: Seva C , et al. Lorglumide and loxiglumide inhibit gastrin-stimulated DNA synthesis in a rat tumoral acinar pancreatic cell line (AR42J). Cancer Res. 50: 5829-5833, 1990. PubMed: 2393852
23152: Rajasekaran AK , et al. Structural reorganization of the rough endoplasmic reticulum without size expansion accounts for dexamethasone-induced secretory activity in AR42J cells. J. Cell Sci. 105: 333-345, 1993. PubMed: 7691838
23222: Longnecker DS , et al. Effect of age on nodule induction by azaserine and DNA synthesis in rat pancreas. J. Natl. Cancer Inst. 58: 1769-1775, 1977. PubMed: 864754
23408: Huang Y , Hui DY . Cholesterol esterase biosynthesis in rat pancreatic AR42J cells. Post- transcriptional activation by gastric hormones. J. Biol. Chem. 266: 6720-6725, 1991. PubMed: 2016288
23412: Menniti FS , et al. Turnover of inositol polyphosphate pyrophosphates in pancreatoma cells. J. Biol. Chem. 268: 3850-3856, 1993. PubMed: 8382679
23421: Logsdon CD , et al. Glucocorticoids increase amylase mRNA levels, secretory organelles, and secretion in pancreatic acinar AR42J cells. J. Cell Biol. 100: 1200-1208, 1985. PubMed: 2579957
23552: Zhao H , et al. Regulation of intracellular Ca2+ oscillation in AR42J cells. J. Biol. Chem. 265: 20856-20862, 1990. PubMed: 1701171
23554: Zhao H , Muallem S . Inhibition of inositol 1,4,5-trisphosphate-mediated Ca2+ release by Ca2+ in cells from peripheral tissues. J. Biol. Chem. 265: 21419-21422, 1990. PubMed: 2174872
23556: Ihara H , Nakanishi S . Selective inhibition of expression of the substance P receptor mRNA in pancreatic acinar AR42J cells by glucocorticoids. J. Biol. Chem. 265: 22441-22445, 1990. PubMed: 1702421
48308: Adell T , et al. Role of the basic helix-loop-helix transcription factor p48 in the differentiation phenotype of exocrine pancreas cancer cells. Cell Growth Differ. 11: 137-147, 2000. PubMed: 10768861
48309: Seva C , et al. Growth-promoting effects of glycine-extended progastrin. Science 265: 410-412, 1994. PubMed: 8023165
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发表于 2012-9-12 12:41 资料 个人空间 短消息  加为好友 

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48311: Negre F , et al. Autocrine stimulation of AR4-2J rat pancreatic tumor cell growth by glycine-extended gastrin. Int. J. Cancer 66: 653-658, 1996. PubMed: 8647628
48312: Bertrand V , et al. Inhibition of gastrin-induced proliferation of AR4-2J cells by calcium channel antagonists. Int. J. Cancer 56: 427-432, 1994. PubMed: 7508895
57433: Mashima H , et al. Betacellulin and activin A coordinately convert amylase-secreting pancreatic AR42J cells into insulin-secreting cells. J. Clin. Invest. 97: 1647-1654, 1996. PubMed: 8601630
57434: Palgi J , et al. Transcription factor expression and hormone production in pancreatic AR42J cells. Mol. Cell. Endocrinol. 165: 41-49, 2000. PubMed: 10940482
90275: Mashima H , et al. Formation of insulin-producing cells from pancreatic acinar AR42J cells by hepatocyte growth factor. Endocrinology 137: 3969-3976, 1993. PubMed: 8756573
90276: Silver K , Yao F . ARIP cells as a model for pancreatic beta cell growth and development. Pancreas 22: 141-147, 2001. PubMed: 11249068
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发表于 2012-9-12 12:41 资料 个人空间 短消息  加为好友 

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Cell Lines

ATCC Number: CRL-1918
Designation: CFPAC-1 Depositors: RA Schoumacher

Biosafety Level: 1 Shipped: frozen

Medium &
Serum: See Propagation Growth
Properties: adherent

Organism: Homo sapiens (human) Morphology: epithelial


Source: Organ: pancreas
Disease: ductal adenocarcinoma; cystic fibrosis


Cellular
Products: carcinoembryonic antigen (CEA), 9 ng/ml; pancreatic oncofetal antigen (POA), 28 ng/ml; adenocarcinoma associated antigen (ACAA), 5000 ng/ml; CA 19-9 antigen, 12000 units/ml; epithelial keratins
Permits/Forms:

In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

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