小中大For your reference:
To my knowledge, to use a buffer in histology experiment, we need to consider:
1. To maintain constant pH during the time of the fixation. The pH of the fixative in EM is adjusted using the buffer system chosen; e.g. range 6.5-8.0.
2. The ionic constitution to prevent the extraction of cellular components, or the deposition of precipitates of fixative chemicals.
To use a buffer in biochemistry experiment, we need to consider much more things, for details refer to the following web site, please.
cuturl('http://www.ipfw.edu/chem/533/533/buffers.htm')
