生物实验技术

实验概要

Rapid and efficient purification of PCR products from salts, primers, dNTPs, and other non-nucleic acid reagents.

实验原理

The ChargeSwitch®Technology
The ChargeSwitch®Technology is a novel magnetic bead-based technology providing a switchable surface that is charge dependent on the surrounding buffer pH to facilitate nucleic acid purification. The ChargeSwitch®chemistry is ideal for purification of DNA using liquid handling robots, avoiding the need for centrifugation steps or the use of ethanol or chaotropic salts. In low pH conditions, the ChargeSwitch®Magnetic Beads have a positive charge and binds the negatively charged nucleic acid backbone (see figure below). Proteins and other contaminants are not bound and are washed away using the wash buffer. To elute nucleic acids, the charge on the surface is neutralized by raising the pH to 8.5 using a low salt elution buffer (see figure below). Purified DNA elutes instantly into this elution buffer.



主要试剂

ChargeSwitch®Purification Buffer (N5)
ChargeSwitch®Magnetic Beads
ChargeSwitch®Wash Buffer (W12)
ChargeSwitch®Elution Buffer (E5)


实验材料

PCR samples
96 x 200 µl U-bottomed microtiter plate
Any liquid handling robotic workstation with a gripper arm to process samples in 96-well plates
Appropriate tips for liquid dispensing and aspiration
96-Well Magnetic Separator
Shaker