小中大相关疾病：
阿尔茨海默病
1. 从你的图中看来，你的resin根本就没有起作用，纯粹就是个筛子，什么蛋白都下来了。因此，你的蛋白很有可能没有挂到柱子上。
2、第二种可能性，你的洗脱液不合适，将靶蛋白和杂蛋白一起洗下来了。
3、还有一种可能性，就是虽然表达的蛋白是可溶性的，但是却像包涵体一样聚集在一起，无法为resin所捕捉。这就很不幸了，请看： About 20% of the MBP fusion proteins we have constructed do not bind efficiently to amylose resin. The reason for this is unclear, but it may be symptomatic of a fusion protein that exists in a soluble but aggregated state wherein the majority of the passenger protein is not properly folded. Sometimes better results can be obtained by performing amylose affinity chromatography at room temperature instead of 4 °C. This problem can also be circumvented by using vectors that are designed to produce MBP fusion proteins with additional accessory tags（具体参考附件内容。）
4、你没有说清楚各个泳道是什么，蛋白也没有标示位置和大小，无法判断是否降解。
