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Trypsin inhibitor
from Glycine max (soybean)
Product Number T 9008
Storage Temperature 2-8 °C
Product Description
CAS Number: 9035-81-8
Extinction Coefficient: E1% = 9.94
(280 nm, pH 7.6 buffer)
pI: 4.51
Synonyms: Kunitz Trypsin Inhibitor, Tia1, STI, and
SBT1
This product is a 1% solution of trypsin in water.
Soybean trypsin inhibitor was first isolated by Kunitz.2
Several other related inhibitors are also found in
soybeans.3 Trypsin inhibitor from soybeans is a
monomeric protein containing 181 amino acid residues
in a single polypeptide chain crosslinked by two
disulfide bridges.4,5,6 The molecular weight
determined from the amino acid sequence is 20.1 kDa.
Soybean trypsin inhibitor inhibits trypsin, and to a
lesser extent chymotrypsin8 and plasmin.9 Soybean
trypsin inhibitor will also inhibit proteases with
mechanisms similar to trypsin. This product will also
inhibit plasma kallikrein and coagulation Factor XA.
However, this product will not inhibit metalloproteases,
tissue-based kallikrein, acid proteases, or thio
proteases.
Soybean trypsin inhibitor forms a 1:1 stoichiometric
complex with the protease active site. Upon formation
of this complex, trypsin may cleave a single arginineisoleucine
bond on the inhibitor.10,11 Inhibition is both
reversible and pH dependent. Dissociation of this
complex may yield a modified or native form of the
inhibitor.12 The optimal pH for trypsin binding is 8.0
with an association constant of greater than 109 at
pH 8.0 and 0.15 - 2.6 x 104 at pH 3.6 to 4.4.13
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以上是在SIGMA 网站上找到的信息，没有看到关于TYPE I II的介绍。可否告知链接？或是英文全名？也许以此做关键词可以找到。
