小中大
多谢楼主！我正好要看这方面的文章，多支持，多鼓励！
我从pubmed上面看到了一篇关于蛋白质组学在心血管系统的应用的文章很好，可惜我看不到全文。高人的话，帮帮忙了，大家共享啊！Dis Markers. 2004;20(3):167-78.
Heart disease, clinical proteomics and mass spectrometry.
Stanley BA, Gundry RL, Cotter RJ, Van Eyk JE.
Department of Medicine, Johns Hopkins University, Baltimore, MD 21224, USA.
Heart disease is the leading cause of mortality and morbidity in the world. As such, biomarkers are needed for the diagnosis, prognosis, therapeutic monitoring and risk stratification of acute injury (acute myocardial infarction (AMI)) and chronic disease (heart failure). The procedure for biomarker development involves the discovery, validation, and translation into clinical practice of a panel of candidate proteins to monitor risk of heart disease. Two types of biomarkers are possible; heart-specific and cardiovascular pulmonary system monitoring markers. Here we review the use of MS in the process of cardiac biomarker discovery and validation by proteomic analysis of cardiac myocytes/tissue or serum/plasma. An example of the use of MS in biomarker discovery is given in which the albumin binding protein sub-proteome was examined using MALDI-TOF MS/MS. Additionally, an example of MS in protein validation is given using affinity surface enhanced laser desorption ionization (SELDI) to monitor the disease-induced post-translational modification and the ternary status of myoctye-originating protein, cardiac troponin I in serum.
PMID: 15502250 [PubMed - in process]
