小中大请问各位高手，我能在一张膜上同时孵育两种抗体吗？因为我的目的蛋白和内参相差不是很远，做了好多次都没有好结果，我在想是不是切胶时没有切好，所以我想试一下不切胶，整块转膜，然后同时孵育抗体。请问这样行吗？如果行的话，抗体怎么配制呢？是不是在一定体积的抗体稀释液中加入两种抗体呢？ 两种抗体的来源有什么要求吗？都是同种属来源的行吧？ 谢谢各位！
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well, it is very important to avoid mixing two different antibodies together.
Personally, I hate stripping as well.
My suggestions will be :
you can 1. ponceau stain your membrane after transfer and cut your membrane according to the protein bands
or2. probe with your protein of interest first, then directly probe with you loading control again. No stripping needed, because your target protein is not overlapping with the loading control. No worries.
Either way worked for me.
