小中大
Go to Hampton Research website to check materials for protein crystallization.
Normally, you need to get pure protein first;
then do crystal screening to see if you could get protein crystal;
then optimize the crystallized conditions to get good single crystal;
then collect X-Ray diffraction data at synchrotron beamline at Beijing or other synchrotron facilities all over the world, or lab-based X-ray equipment;
then solve the protein structure by softwares(if you really want to do the thing, be familiar with softwares, such as CCP4, CNS, SHELX, COOT, solve/resolve and others, from now on. Some softwares are free to academia so you can download for their websites, but some not free, if you boss has enough money, just ask to buy, but I think many bosses in China are miser).
then deposit you structure to PDB (protein data bank), and publish your paper if you get the structure. If you can do the relative functions, that is better. Structure plus function is the best. A very good paper will you get.
Anyway, the experiments are routine. You have to spend more time on softwares if you want to be an expert.
Besides, I think you need to read some textbooks about protein crystallography. Basic knowledge is necessary.
