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ProtParam
User-provided sequence:
10 20 30 40 50 60 CDLPQTHSLT HTRALRLLAQ MRRISPFSCL DHRRDFGFPQ EALGGNQVQK AQAMALVHEM 70 80 90 100 110 120 LQQTFQLFST EGSAAAWDES LLHQFCTGLD QQLRDLEACV MQEAGLEGTP LLEEDSILAV 130 140 150 160 RKYFHRLTLY LQEKNYSLCA WEIIRAEVMR VFSSSTNLQD TLRKKE References and documentation are available.
Please note the modified algorithm for extinction coefficient.
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Number of amino acids: 166
Molecular weight: 19058.7
Theoretical pI: 5.87
Amino acid composition: Ala (A) 14 8.4%
Arg (R) 12 7.2%
Asn (N) 3 1.8%
Asp (D) 8 4.8%
Cys (C) 5 3.0%
Gln (Q) 15 9.0%
Glu (E) 13 7.8%
Gly (G) 7 4.2%
His (H) 6 3.6%
Ile My heart 4 2.4%
Leu (L) 25 15.1%
Lys (K) 5 3.0%
Met (M) 5 3.0%
Phe (F) 8 4.8%
Pro (P) 4 2.4%
Ser (S) 11 6.6%
Thr (T) 10 6.0%
Trp (W) 2 1.2%
Tyr (Y) 3 1.8%
Val (V) 6 3.6%
Pyl (O) 0 0.0%
Sec (U) 0 0.0%
(Black Eye 0 0.0%
(Z) 0 0.0%
(X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 21
Total number of positively charged residues (Arg + Lys): 17
Atomic composition:
Carbon C 836
Hydrogen H 1323
Nitrogen N 239
Oxygen O 251
Sulfur S 10
Formula: C836H1323N239O251S10
Total number of atoms: 2659
Extinction coefficients:
Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.
Ext. coefficient 15720
Abs 0.1% (=1 g/l) 0.825, assuming ALL Cys residues appear as half cystines
Ext. coefficient 15470
Abs 0.1% (=1 g/l) 0.812, assuming NO Cys residues appear as half cystines
Estimated half-life:
The N-terminal of the sequence considered is C (Cys).
The estimated half-life is: 1.2 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 56.56
This classifies the protein as unstable.
Aliphatic index: 87.05
Grand average of hydropathicity (GRAVY): -0.314
疏水性为-0.314，表示疏水性弱。但是这和能否用疏水柱进行纯化没有多强的关系。
