小中大
BL21(DE3)
F– ompT gal dcm lon hsdSB(rB- mB-) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5])
an E. coli B strain with DE3, a λ prophage carrying the T7 RNA polymerase gene and lacIq
Transformed plasmids containing T7 promoter driven expression are repressed until IPTG induction of T7 RNA polymerase from a lac promoter.
Derived from B834 (Wood, 1966) by transducing to Met+.
See the original Studier paper or the summary in Methods in Enzymology for more details.
DH5α
F- endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG Φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17(rK- mK+), λ–
An Hoffman-Berling 1100 strain derivative (Meselson68)
Promega also lists phoA
nalidixic acid resistant
References:
FOCUS (1986) 8:2, 9.
Hanahan, D. (1985) in DNA Cloning: A Practical Approach (Glover, D.M., ed.), Vol. 1, p. 109, IRL Press, McLean, Virginia.
Grant, S.G.N. et al. (1990) Proc. Natl. Acad. Sci. USA 87: 4645-4649 PMID 2162051.
Meselson M. and Yuan R. (1968) Nature 217:1110 PMID 4868368.
详见 cuturl('http://openwetware.org/wiki/E._coli_genotypes#BL21.2E3.29')
至于质粒的信息，网上多如牛毛，详见 cuturl('http://www.google.com/advanced_search?hl=en')
