小中大Probably too much nucleic acids clogged the column. Try to use 0.1% PEI to remove nucleic acids and then ammonium sulfate precipitate your target protein, next dialyze against your his-tag binding buffer and then load onto the column.
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请问核酸会引起蛋白不稳定？ 为什么要用PEI沉淀, 用核酸酶不行吗？为什么要硫酸铵沉淀？能不能解释下？谢谢！
