小中大I am very appreciated for your reply. You are just a well establised example for me! A paper in the Journal of Nature Medicine requires much and much hardwork. So what I want to do is work work and work. Try my best and make fully of myself!
Because the sample is hard to get, It would be better to choose in-solution digestion and then the sample run directly on the SCX-RPLC-LTQ-MS/MS or SCX-RPLC-LTQ-FT-MS/MS. However, without prefraction(SDS or IEF-SDS) maybe only a small number of proteins would be indentified.
In theory, FT is more sensitive than LTQ, isn't it?
It's so sad that I have been preparing for my sample for months!
We can communicate sometime.
iTRAQ对质谱仪有什么特殊要求吗？有相关的生物信息处理软件吧。这iTRAQ标记技术不是很成熟，效率可能不如乙酰化。在这只在标准样品做过，复杂样品没人做，我的样品将会很宝贵，所以正在考虑。
