小中大请问一个关于检测限(LOD)和定量限(LOQ)的问题
请问一个关于检测限(LOD)和定量限(LOQ)的问题
我的一篇文章投到analytical chimica acta之后,编辑一直问一个关于LOD和 LOQ的问题。我定义了检测限为3倍信噪比,定量限为10倍信噪比。我做的时候是标准样品倍比稀释,浓度分别为40, 20, 10, 5, 2.5μg/kg,然后用HPLC检测,得到的LOD5μg/kg,为LOQ10μg/kg。但是编辑认为我既然定义了检测限为3被信噪比,定量限为10被倍信噪比,那么定量限应该为检测限的3.3倍,可是我的浓度本来就是倍比稀释的啊,信噪比大于10的最低浓度为10μg/kg,信噪比大于3的最低浓度为5μg/kg。
我不知道怎么解释他们才能听懂,还是我应该改变检测限和定量限的定义呢?着急,谢谢好心人!
如下是编辑的comments
In your response to my comments you have written "We defined LOD as the lowest drug concentration in the samples can be measured with a signal to the background signal (S/N) ratio of 3/1(S/N), and LOD as the lowest drug concentration in food samples can be measured with a signal to the background signal (S/N) ratio of 10/1(S/N). After the detection, we found that the LOD was 5 <mu>g/kg and the LOQ was 10 <mu>g/kg." If 5 µg/kg gives a signal ise ratio of 3/1, 10 µg/kg cannot give a signal ise ratio of 10/1 (it would give just a signal ise ratio of 6/1) !!! Either your value for the LOD or your value for the LOQ is wrong.