小中大
Date accepted: 03/02/1985
Other name: BALB3T3
Animal: mouse
Strain: BALB/c
Scientific Name: Mus musclus
Case history: normal
Tissue prepared: embryo
History of the cell: Arronson & Todaro A31 clone BALB 3T3 A31-1-1
Medium: Eagle's minimal essential medium with 10% fetal calf serum
Passage method: 0.25% trypsin & 0.02% EDTA. Practically, 1/100 dilution once a week. Avoid being confluent.
CO2 Concentration: 5 %
Cell no. at passage: 3x10^5 cells/60 mm dish
Genetics: whole embryo
Life span: infinite
Characteristics: contact inhibited mouse cell line
Established by Kakunaga,T.
Deposited by Kuroki,T.
Cell bank: TIMS(JCRB)
References: (1,2,3,4)
Comments: The FCS requires careful selection to keep quality.
(* The format of the table was revised on Apr.25.2003.)
