小中大参 考 文 献 [1] B eteH .Ch emical&EngineeringN ews.20 02,80(19):33. [2]M ottramD S ,et al .Ac rylamideis fo rmedin th eM ailardr eaction[J ].Natur e,2 00 2,419:448. [3]St adlerR H ,et al .A crylamidefro mM ailardre actionp roducts[J I.N ature, 20 02 ,4 19:449 仁4]IA RC.Mo nographson th eev aluationof ca rcinogenri skto hu mans:so mein - dus tri alc h emicals.N o.60 .Ly on:in ternationala gencyf orr esearcho nc ancer ,1 9 9 4.389一433. 仁5] W TO.Gu idelinesfo rd rinkingw aterq uality[M I.Second.Ce neva,19 93. (1) :7 3. [6]F DAC entersfo rd iseasec ontrolan dp revention.Ex ploratory datao na crylami de in fo ods一February2 003u pdate[EB /0L].cuturl('http://www.ofs') an.fda . gov /一 山 ns. [7] T akatsukiS ,Ne motoS ,Sa sakiK ,et al .De terminationo fac rylamidein p roces sed fo o ds场LC/MSu singc olumns witching[JI . ShokuhinE iseigaku Zas sh i.2 003,44(2):8 9一95. [8)Sh arpD .Acrylamidein fo od[J].L ancet,2003,361:361. (收 稿 日 期 :20 04- 07 -04 ) 【研究报告】 〔文章编号〕1004-8685(2004)06-693-03 沉淀缓冲增菌法提高水中ElTo :霍乱弧菌阳性检出率的研究和应用‘ 陈德云，张慧红，胡伟宁，陈国雄，符发雄，巫结冰，老倩群 (佛山市顺德区疾病预防控制中心，广东528300) 摘 要 〔目的〕应用化学沉淀剂将ElT or霍乱弧菌沉淀聚集于水体下层，在缓冲溶液作用下保持特定的生长环境， 从而起到抑制其它肠杆菌的生长，避免和减少检测中易出现假阴性的误差，以达到提高霍乱弧菌的阳性检出率。为疫情 监测和调查提供准确可靠的科学依据。〔方法〕以碳酸钠为介质，硫酸亚铁为沉淀剂，在一定比例用量下，沉淀剂将水中 霍乱弧菌沉淀聚集于水体下层，弃去上层清液沉淀中加入碳酸盐缓冲溶液。用等量双倍浓碱蛋白陈进行增菌、分离、鉴 定。(结果〕沉淀剂可将水中97.67%一99.85% El Tor霍乱弧菌沉淀至水体下层，缓冲溶液在增菌过程中声变异数 1.19%，处于不变的良好状态。在此条件下El Tor霍乱弧菌最小检量0.014 - 0.140cfu/ml，比常规增菌法阳性检出提高一 个对数;沉淀缓冲增菌法比常规增菌法阳性检出率提高2.6倍。检测中无需二次增菌，比常规增菌法缩短一半的工作周 期，同时可降低节约70%的试剂成本。〔结论〕本检验方法具有操作简便，精确度高，最小检出量大，阳性检出率高，工作 周期短，试剂成本低的特点，适用于霍乱传染病的预防控制和调查。 关键词霍乱弧菌;沉淀缓冲;阳性检出率 Study and application of precipitation bufering proliferation method to enhance positive rate of El Tor vibrio cholerae in water ChenD eyun，ZhangH uihong,Hu W eining,et al .ShundeC enterfo rD iseaseC ontrolan d an was used to precipitate El Tor Vihrio Prevention, Guangdong 528300, China. (Objective) Chemical p cholerae to the s}ilgtrate of water.玩bufer sogrowth conditions were maintained, to restrain positive rate of El Tor Vibrio cholerae the growth enhanced. of other Escherichia Colt and reduce determination error of false Accurate scientific basis was provided for monitoring and survey Methods) In sodium carbonate medium, ferrous sulfate of certain ratioquantity precipitated and accumulated El Tor Vibno With lucid water taken away, carbonate was added to as bufer solution. After then, alkaline peptone of concentrationw asa ddedw itht hes amec ubageto p roliferate，separatea ndid entifyE lTo rVi bnoc holerae.(Re sults)P recipitator ， 基金项目:广东省佛山市卫生局医学科研立项，佛卫(2004)226号(项目编号:2004472) 万方数据
