液质联用

有高手做过么？请给一些指导性意见。谢谢！

环丙沙星等喹诺酮类药物用液质来作应该不难，建议：

1）查找相关文献看一下
2）买标准品，在自己的仪器上Tune，自己作方法。

不同仪器，不同实验室作出来，条件可能会不一样。
Good luck！

下面三篇文献你可能用得上，自己去找吧！

1）Tuerk J, Reinders M, Dreyer D, Kiffmeyer TK, Schmidt KG, Kuss HM. Related Articles, Links
Analysis of antibiotics in urine and wipe samples from environmental and biological monitoring--comparison of HPLC with UV-, single MS- and tandem MS-detection.
J Chromatogr B Analyt Technol Biomed Life Sci. 2006 Feb 2;831(1-2):72-80. Epub 2005 Dec 9.
PMID: 16338183 [PubMed - indexed for MEDLINE]

2）Hatano K. Related Articles, Links
[Simultaneous determination of quinolones in foods by LC/MS/MS]
Shokuhin Eiseigaku Zasshi. 2004 Oct;45(5):239-44. Japanese.
PMID: 15678937 [PubMed - indexed for MEDLINE]

3）Volmer DA, Mansoori B, Locke SJ. Related Articles, Links
Study of 4-quinolone antibiotics in biological samples by short-column liquid chromatography coupled with electrospray ionization tandem mass spectrometry.
Anal Chem. 1997 Oct 15;69(20):4143-55.
PMID: 9337593 [PubMed - indexed for MEDLINE]