液相色谱

各位有没有做过普拉格雷的啊？我一个客户在做，但不知道用什么色谱柱……

用C18柱肯定能做！放心吧，看了下结构并不是什么难分析的物质！

C18柱，色谱条件要麻烦些，它有异构体！

根据物质的结构和性质来选

这个要确实国内很少有人在做，主要是活性代谢产物起作用  你用串级质谱就行啊 那样就不用考虑色谱柱问题啊 内标用氯吡格雷就行了

万能的VIP群主啊~~~你说色谱条件麻烦些，那你能告诉下我到底是啥麻烦条件吗？你要是告诉我了，我让miracle把我能加的分都给你都行~~~

以下资料或许对你会有帮助！

Chromatographic separation was achieved on an Inertsil ODS-3

column (particle size 5_m, 2.1mm×50mm, GL Sciences, Tokyo)

at a column temperature of 40 ◦C using amobile phase of methanol

and 1% formic acid (70:30, v/v) at a flow rate of 0.2 mL/min. The

sample injection volume was 10_L and the analytical run time

was 6 min.
上边是氯吡格雷
Monochrom C18 analytical
column (2mm
50mm, 5 mm, Varian Canada, Inc., Mississauga,
Ontario, Canada) was used and the analytes were
separated under isocratic conditions using a flow rate of
0.25 mL/min and a column temperature of 58C. The mobile
phase consisted of 1% formic acid/methanol 50:50 (v/v) and
46:54 (v/v) for the inactive metabolites assay and active
metabolite assay, respectively.

这是普拉格雷

Determination of the active and inactive metabolites
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Quantitative determination of clopidogrel active
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miracle 啊，劳您大驾，把我能给的分数都给haohaorenjia同学吧，他回答的太专业了，非常有价值~~~~  haohaorenjia，多谢你哈，也包括所有回复的人，祝你们出门都捡一个大钱包~~~