小中大
谈一下个人总结破骨细胞的内容,仅供楼主参考:
1 破骨细胞的发育来源
破骨细胞的发育来源较为明确,是造血干细胞分化为单核/巨噬细胞后在多种细胞因子 (TNF-a、RANKL、RANK、OPG、M-CSF、PGE2等)的调控下分化为成熟的具有多个细胞核的巨细胞 。
2 破骨细胞的鉴定
破骨细胞呈特异性的抗酒石酸的酸性磷酸酶染色阳性(TRAP+),F-actin染色阳性,表达降钙素受体、组蛋白酶K(cathepsin-k)、整合素αvβ3(integrinsαvβ3),内可以分解骨组织,体外培养可以在骨片、象牙或者人工骨组织培养板上可以形成吸收性陷窝。
3附上几篇关于破骨细胞鉴定的较为权威具体的文献
(1) Chambers, T. J. Regulation of the differentiation and function of osteoclasts. J. Pathol, 2000; 192: 4–13
(2) Boyle, W.J., Simonet, W.S. & Lacey, D.L. Osteoclast differentiation and activation. Nature, 2003; 423: 337–342
(3)Udagawa, N. Takahashi N, Akatsu T,et al. Origin of osteoclasts: mature monocytes and macrophages are capable of differentiating into osteoclasts under a suitable microenvironment prepared by bone marrow-derived stromal cells. Proc. Natl Acad. Sci. USA, 1990;87:7260–7264
(4) Lee SH, Rho J, Jeong D, et al. v-ATPase V0 subunit d2–deficient mice exhibit impaired osteoclast fusion and increased bone formation Nat Med. , 2006; 12:1403 - 1409
(5) Teitelbaum, S., L., and F. P. Ross. Genetic regulation of osteoclast developmentand function. Nat. Rev. Genet,2003;, 8: 638–649.
(6)Teitelbaum, S.L. Bone resorption by osteoclasts. Science,2000;289:1504–1508
4小鼠的破骨细胞TRAP+照片(×100)