小中大推荐使用QuantiScan,
这是其主页上的介绍。下载到处都有，谈杰和hoyoyo上的都有。
**，hoyoyo上有。我放了一个到yahoo公文包，具体说明在bioon上。
QuantiScan has similar functionality to sophisticated densitometers but at a fraction of the cost. It is able to analyze a wide variety of material including polyacrylamide and agarose gels, autoradiograms, TLC plates, etc. The program will accept images for analysis directly from any TWAIN compliant scanner as well as most uncompressed .TIF or .BMP files. For laned gels of reasonable quality, the analysis is highly automated allowing extremely high throughput of data. Almost any image can be analyzed with the manual modes of QuantiScan.
Images can be preprocessed by rotation, horizontal or vertical flip, cropping to remove background and inversion or re-calibration of grayscale. Also, during the preprocessing stage, there is an option to calibrate lanes for a known standard solution which allows automatic calculation of mol. wts of unknowns. In addition, if lanes on gels are known to be precisely spaced, there is a facility to simply specify their total number and whether they are to be measured one or two-dimensionally.
For lane analysis, lanes of any width can also be created manually or by using the unique automatic lane drawing mode. This detects the edges of each lane and thus allows for irregular spacing and width. Lanes can be skewed to allow for gel imperfections. Lanes can also be copied and the copies moved with the mouse or cursor keys. Once lanes have been drawn, a graph of density versus position is automatically produced. If background subtraction has been opted for, the graph also displays this. Background subtraction methods include: Minimum, Interpolated Minimum, Valley, Edge and Polynomial. The density level of user-selected areas of images can also be displayed as 3-D density maps which can be printed, saved or copied to the clipboard.
Densitometric peaks for each lane can be specified manually or automatically with the sophisticated peak search routine. Optionally, each peak can be labelled on the graph to make lane comparisons easier. Where appropriate, automatic fitting of Gaussian peaks can also be performed. This automatic fitting uses a least-squares minimization technique to fit a sequence of peaks and a polynomial background to the given profile. Peak areas are calculated by integration using Simpson's rule and, if the automatic fitting option has been used, the area of the fitted peak is also calculated and displayed.
As well as the calibration to a known standard solution, mentioned above, there is the ability to manually select standard peaks on the densitometry graph and calibrate their mol. wts or other units to their position on the X-axis with a variety of models including linear, logarithmic and exponential. When this has been done, the table of results automatically displays the fitted values of the standard and test peaks. Reports are also generated for the other peak parameters, including position, height, width, background and net area. Together with the graphical results, these can be printed, saved to disk or copied to the clipboard for transfer to other programs. A facility is available to select one lane on a gel as a standard and this can then be compared with other lanes by superimposing the graphs and presenting results for the standard lane alongside each test lane. The parameters of the standard lane can also be saved in a file and recalled for comparisons between different images.
QuantiScan also offers a separate mode to analyze discrete blots. The blots can be detected automatically or specified manually by creating a rectangle around each with the mouse. Any rectangle can be duplicated and the copy moved over another blot with the mouse or cursor keys to ensure that the area of background remains constant. Blot number, position, height, area and densitometric volume are automatically reported. Background subtraction is optional. Numeric, graphical and image output can be printed as reports and can also be saved as files or copied to the clipboard for easy transfer to other programs. Calibration can be done automatically in this mode, using standard solutions. In the case of blots, it is densitometric volume which is the basis of calibration, enabling use of solutions of known concentration as standards for assay purposes.
QuantiScan is compatible with any version of Windows from 3.1 onwards.
