小中大
楼主所用的引物在DNA和RNA中均能扩出来,下面这个是以DNA为模板扩出的片段,长度=91574-86987+1(bp)=4588bp
gi|3687208|gb|AC005742.1| Mus musculus chromosome 11, BAC clone CT7-111I18 (LBNL M01),
complete sequence
Length=159500
Score = 44.1 bits (22), Expect = 0.017
Identities = 22/22 (100%), Gaps = 0/22 (0%)
Strand=Plus/Plus
Query 19 TCGAAAAGCCCGAAAGAGTCTC 40
||||||||||||||||||||||
Sbjct 86987 TCGAAAAGCCCGAAAGAGTCTC 87008
Score = 40.1 bits (20), Expect = 0.27
Identities = 20/20 (100%), Gaps = 0/20 (0%)
Strand=Plus/Minus
Query 1 TCGGCATTTTGAACGAGGTC 20
||||||||||||||||||||
Sbjct 91574 TCGGCATTTTGAACGAGGTC 91555
下面这个是以RNA为模板扩出的片段,长度=379-162+1(bp)=218bp >gi|51826|emb|X03532.1|MMIGG1R Mouse mRNA for IgG1 induction factor
Length=580
Score = 44.1 bits (22), Expect = 0.017
Identities = 22/22 (100%), Gaps = 0/22 (0%)
Strand=Plus/Minus
Query 19 TCGAAAAGCCCGAAAGAGTCTC 40
||||||||||||||||||||||
Sbjct 379 TCGAAAAGCCCGAAAGAGTCTC 358
Score = 40.1 bits (20), Expect = 0.27
Identities = 20/20 (100%), Gaps = 0/20 (0%)
Strand=Plus/Plus
Query 1 TCGGCATTTTGAACGAGGTC 20
||||||||||||||||||||
Sbjct 162 TCGGCATTTTGAACGAGGTC 181