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Title：Molecularly Imprinted Solid-Phase Extraction of Diazepam and Its Metabolites from Hair Samples
题目：分子印迹固相萃取毛发样品中的地西泮及其代谢物
Author： Marinah M. Ariffin, Eleanor I. Miller, Peter A. G. Cormack, and Robert A. Anderson
作者：Marinah M. Ariffin, Eleanor I. Miller, Peter A. G. Cormack, and Robert A. Anderson
Resource: Analytical Chemistry, 2007，79 (1)： 256 -262.
来源：《分析化学》2007年79卷第1期256-262页
Abstract：
摘要：
An anti-diazepam, molecularly imprinted polymer (MIP) has been synthesized and used to extract diazepam and other benzodiazepines from hair samples via a molecularly imprinted solid-phase extraction (MISPE) protocol.
一种抗地西泮的分子印迹聚合物(MIP)已经合成，并将分子印迹固相萃取(MISPE)技术用于从毛发样品中提取地西泮（成分）和提取其他地西泮（药物)。
Optimum retention of diazepam on the MIP columns was achieved using an apolar solvent, and the binding capacity of the polymer toward diazepam was found to be 110 ng of diazepam/mg of polymer.
在分子印迹聚合物柱上，使用非极性溶剂后，地西泮的保留良好可以实现，并且发现地西泮聚合物的结合容量是110ng地西泮/毫克聚合物。
The recovery of a 50 ng diazepam standard spiked into blank hair was 93%, with good precision (RSD = 1.5%). The LOD and LOQ of diazepam in spiked hair samples were 0.09 and 0.14 ng/mg, respectively.
添加到空白头发中的50ng地西泮样品回收率是93%,且有好的精密度 (RSD为1.5%)。在添加的头发样品中地西泮检测限和定量限分别为0.09和0.14纳克/毫克。
The MISPE method was demonstrated to be applicable to the analysis of diazepam metabolites and other benzodiazepine drugs, in addition to diazepam itself.
分子印迹固相萃取方法（MISPE）被证明了可用于地西泮代谢物和其他苯二氮卓类药物的分析，但除地西泮外。
The application of the extraction method to postmortem hair samples yielded results that were in good agreement with the corresponding ELISA data (from blood samples) and data arising from the analysis of the same blood samples using a validated in-house SPE-LC-MS-MS method.
死后头发样本提取法的应用已取得了结果,该方法是与从血液样品中（得到的）相应ELISA数据和运用一种有效的内部 SPE-LC-MS-MS方法从相同的血液样品中分析（得到）的数据一致。
