小中大How do I know which medium to use with my particuliar cell line?
There are no set culture conditions for growing a certain cell type. A culture grown in MEM, can probably be just as easily grown in DMEM or Medium 199. In general a good place to start is MEM for adherent cells and RPMI-1640 for suspension cells. AIM V (12055) is a good choice as an all-purpose serum-free medium (SFM). The conditions listed below can be used as a guide line when setting up a new culture.
CELL LINE CELL TYPE SPECIES TISSUE MEDIUM
293 fibroblast human embryonic kidney MEM, 10% HI horse serum
3T6 fibroblast mouse embryo DMEM, 10% FBS
A549 epithelial human lung carcinoma F-12K, 10% FBS
A9 fibroblast mouse connective tissue DMEM, 10% FBS
AtT-20 epithelial mouse pituitary tumor F-10, 15% horse serum and 2.5% FBS
BALB/3T3 fibroblast mouse embryo DMEM, 10% FBS
BHK-21 fibroblast hamster kidney GMEM, 10% FBS or MEM, 10% FBS and NEAA
BHL-100 epitheliall human breast McCoy'5A, 10% FBS
BT fibroblast bovine turbinate cells MEM, 10% FBS and NEAA
Caco-2 epithelial human colon adeno carcinoma MEM, 20% FBS and NEAA
Chang epithelial human liver BME, 10% calf serum
CHO-K1 epithelial hamster ovary F-12, 10% FBS
Clone 9 epithelial rat liver F-12K, 10% FBS
Clone M-3 epithelial mouse melanoma F-10, 15% horse serum and 2.5% FBS
COS-1 fibroblast monkey kidney DMEM, 10% FBS
COS-3 fibroblast monkey kidney DMEM, 10% FBS
COS-7 fibroblast monkey kidney DMEM, 10% FBS
CRFK epithelial cat kidney MEM, 10% FBS and NEAA
CV-1 fibroblast monkey kidney MEM, 10% FBS
D-17 epithelial dog osteosarcoma MEM, 10% FBS and NEAA
Daudi lymphoblast human blood from a lymphoma patient RPMI-1640, 10% FBS
GH1 epithelial rat pituitary tumor F-10, 15% horse serum and 2.5% FBS
GH3 epithelial rat pituitary tumor F-10, 15% horse serum and 2.5% FBS
H9 lymphoblast human T-cell lymphoma RPMI-1640, 20% FBS
HaK epithelial hamster kidney BME, 10% calf serum
HCT-15 epithelial human colorectal adenocarcinoma RPMI-1640, 10% FBS
HeLa epithelial human cervix carcinoma MEM, 10% FBS and NEAA (in suspension, S-MEM)
HEp-2 epithelial human larynx carcinoma MEM, 10% FBS
HL-60 lymphoblast human promyeolocytic leukemia RPMI-1640, 20% FBS
HT-1080 epithelial human fibrosarcoma MEM, 10% HI FBS and NEAA
HT-29 epithelial human colon adenocarcinoma McCoy's 5A, 10% FBS
HUVEC endothelial human umbilical cord F-12K, 10% FBS and heparinat 100 ug ml