小中大那就要看你的目的了，如果是想胞内染色法检测样品中已有的Th17，需要用PMA+离子酶素刺激，不需要anti-CD28。
如果是想体外诱导Th17，就需要naive CD4 T+anti-CD3+anti-CD28+IL-6+TGF-β。
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老师，我查到一篇“The sorted cells were primed for 96 h with
anti-CD3 (10 lg/ml; 145-2C11; BD Biosciences) and soluble anti-
CD28 (2 lg/ml; 37.51; BD Biosciences). IL-6 (20 ng/ml), human
TGFb (3 ng/ml), and IL-23 (6 ng/ml) (all from R&D Systems, Minneapolis,
MN) were added to the culture for 4 days to drive the differentiation
of Th17 cells.”但是这是提Th17的，我只想测IL-17，只要抗CD3
、CD28的可以不？谢谢！
