美国细胞、菌种保藏中心American Type Culture Collection (ATCC)
目前它可以提供以下物品：细胞系（3000种）；细菌和噬菌体（15000种）；动植物病毒（2500种）；原生动物 1200种以及重组物品等。(700人癌细胞系
The main repository of cell lines in the United States. Get information on established cell lines and order cells.ATCC is a global nonprofit bioresource center that provides biological products, technical services, and educational programs to private industry, government, and academic organizations around the world.
ps: The STC-1 cell line was derived from an intestinal endocrine tumor in a double-transgenic mouse carrying the rat insulin promoter linked to the simian virus 40 large T antigen and to the polyoma virus small t antigen作者: junhun 时间: 2012-6-30 17:26
Critical Evaluation of ECV304 as a Human Endothelial Cell Model Defined by Genetic Analysis and Functional Responses: A Comparison with the Human Bladder Cancer Derived Epithelial Cell Line T24/83
Julia Brown, Sarah J. Reading, Sarah Jones, Caroline J. Fitchett, John Howl, Ashley Martin, Clare L. Longland, Francesco Michelangeli, Yuri E. Dubrova, and Colin A. Brown
Molecular Pharmacology Group (JB, SJ, CJF, JH, AM, CAB, School of Health Sciences, University of Wolverhampton, Liver Sciences Unit (SJR), Kings College, London, The Rayne Institute, London, School of Biochemistry (CLL, FM), University of Birmingham, Edgbaston, Birmingham, and Department of Genetics (YED), University of Leicester, University Road, Leicester, United Kingdom
SUMMARY: Early reports indicated that ECV304 was a spontaneously-transformed line derived from a Japanese human umbilical vein endothelial cells (HUVEC) culture. Many morphological, immunochemical, and genetic studies provided further evidence that ECV304 was a valuable biomedical research tool and could be used to study processes that include angiogenesis in vitro and signal transduction by a variety of G protein-coupled receptors. However, several distinct differences between ECV304 and HUVEC are now apparent and recent reports have indicated genetic similarity between ECV304 and T24/83, a human bladder cancer cell line. To further assess the utility of ECV304 as a human endothelial cell model, we compared the functional responses of ECV304 and T24/83 to a range of G protein-coupled receptor agonists. We also used DNA fingerprinting to karyotype both ECV304 and T24/83. Both ATP and uridine triphosphate (UTP) stimulated inositol phosphate metabolism in ECV304 without alteration of cAMP levels. Comparative data using selective P2Y receptor agonists indicated that this response, leading to calcium mobilization from intracellular stores, was predominantly mediated by the activation of P2Y2 receptors. Similar responses were recorded from both ECV304 and T24/83 cells. ECV304 expressed a relatively high basal activity of NOS that was reduced by L-NAME and stimulated by P2Y2 receptor agonists. In contrast, P2Y2 receptor activation did not induce prostaglandin synthesis in ECV304. Both ECV304 and T24/83 express receptors for adenosine, adrenaline, and calcitonin, which stimulate adenylate cyclase. Proliferation of ECV304 and T24/83 cells, measured by the incorporation of [3H]thymidine into DNA, was largely serum-independent. This was in contrast to parallel experiments with porcine and bovine aortic endothelial cells that indicated a marked serum-dependent increase in DNA synthesis. Genetic analysis confirmed that ECV304 and T24/83 are identical. ECV304 displays some endothelia characteristics and is useful for the study of receptor pharmacology. However, ECV304 is not of HUVEC origin and is therefore an inappropriate cell line to study endothelial cell biology.CV304细胞系，据说已证实不是人脐静脉内皮细胞系，不知各位有何见解？
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Sincerely,作者: 831226 时间: 2012-7-2 14:57
ScienCell Resesarch Laboratories is the leader in providing normal human cell systems for experimental and therapeutics use in the world. Please visity our website at cuturl('http://www.sciencellonline.com') for detail. The company has distributor in Beijing (Tel. 01051177251, Beijing Qingyuan Bio-Co.). If you have any normal human cell culture related questions, you can visit our website and send us email of your question, our scientific staff will answer your question immediately.作者: 33号 时间: 2012-7-2 14:57
We have many kind of human endothelial cells. Please visit our website at cuturl('http://www.sciencelloline.com') and click"endothelial cells" for detail.作者: zzzz 时间: 2012-7-2 16:06
