国外细胞株资料:
DCTDC Tumor Repository
NCI Frederick Cancer Research & Development Center
Frederick, Maryland (MD)
21702 USA
DSMZ Human and Animal Cell Cultures
German Collection of Microorganisms & Cell Cultures
Mascheroder Weg 1b
Braunschweig (DE)
D-38124 Germany
此细胞库的细胞株似乎不出售到中国!
ECACC European Collection of Cell Cultures
CAMR Centre for Applied Microbiology & Research
Porton Down
Salisbury, Wiltshire (UK)
SP4 0JG UK 作者: kuohao17 时间: 2012-9-12 16:29
中国总代理:广东合川医药有限公司
电话:020-33937770 84105261 传真:020-84105230
电邮:billma79@yahoo.com.cn
ICLC Interlab Cell Line Collection
Istituto Nazionale per la Ricerca sul Cancro c/o CBA
Largo Rosanna Benzi, 10
Genova (GE)
16132 Italy
Phone: +39-0105737 474
Fax: +39-0105737 295
cuturl('http://www.istge.it')
E-mail: iclc@ist.unige.it
中国区域总代理:广东合川医药有限公司
电话:020-33937770 84105261 84105230
电邮:billma79@yahoo.com.cn
网址:cuturl('www.gd-hc.com')
International Histocompatibility Workshop
Istituto Nazionale per la Ricerca sul Cancro c/o CBA
CBA Largo Rosanna Benzi, 10
Genova (GE)
16132 Italy
The cell lines are grown in antibiotic free medium for at least two passages in the quarantine area. Three methods are used to detect mycoplasma infection: DNA staining by Hoechst 33258, PCR amplification and MycoTect assay
DNA staining
The fluorochrome bisbenzimide (Hoechst 33258) binds specifically to the Adenine-Thymine (A-T) regions of DNA. Uninfected cell cultures observed under fluorescence microscopy (image on the left) are seen as fluoresceing nuclei against a negative background; cultures contaminated with mycoplasma (image on the right) appear as brightly fluorescent uniformly shaped bodies surrounding the cells, or as small clusters between the cells.
Artifacts may be fluorescent and interfere with the interpretation of results, but they will appear larger in size than mycoplasma and irregular in shape. The main advantages of this staining method are the speed at which results are obtained and the fact that non-cultivable M. hyorhinis strains can be detected.
Identification of cell lines
Species confirmation
The species of origin of animal cell lines is determined or confirmed by isoenzyme analysis (AuthentiKit TM System, Innovative Chemistry). The electrophoretic mobility of at least two different isoenzymes from a panel of seven (AST, G6PD, LD, MD, MPI, NP, and Pep is determined for each cell line. Recently, Banca Cellule has published a PCR-based method for easily identifying or confirming the species of origin of cell lines by using a panel of oligonucleotides specific for the nine animal species most common in cell culture laboratories (human, cat, dog, mouse, rat, horse, rabbit, African Green monkey and Chinese hamster). Furthermore Banca Cellule has developed a multiplex PCR method, which allow for confirmation of the species of a cell line and for identification of a possible inter-species cross-contamination by a single assay.
STR Identification
Recently, the multiplex short tandem repeat (STR) profiling test was developed for identification of human cell lines. Using this approach, a number of STR loci are amplified by means of PCR with a set of primers, and the products are simultaneously analysed with technologies of automated revelation. This results in a numerical line-specific code, extremely reproducible, which will become integral part of the cell line identification code. The STR test was already carried out on a panel of Banca Cellule cell lines, and will be extended to all the human cell lines.作者: dotaaa 时间: 2012-9-12 16:50