NaCO3(PH11),在此 High-pH下超速离心即可获得细胞膜片,再用Tris-HCl洗去痕量的可溶性蛋白。另外,高pH的处理可以 disrupts sealed membrane compartments without
solubilizing or denaturing the lipid bilayer to allow mapping of the soluble domains of integral membrane proteins,结合LC MS/MS就能基本定位内膜蛋白的“真实”位置。
NaCO3(PH11),在此 High-pH下超速离心即可获得细胞膜片,再用Tris-HCl洗去痕量的可溶性蛋白。另外,高pH的处理可以 disrupts sealed membrane compartments without
solubilizing or denaturing the lipid bilayer to all ...
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(iv)Rickwood. D等Anal, Biochem, 187,318,1990
(v)余兴明"离心技术"设备与方法1993
(VI)余兴明"亚细胞构造的离心分离"--概论,200作者: abc816 时间: 2013-4-23 17:47