good discussion, Thanks a lot!!
Here is my point of view, proteomics per se is just a method, that's it, and to be honest, it is not that mature at all, so that's why proteomics itself becomes a hot area for research. Biological validation of your proteomics data like RT-PCR, western, or even genomics surely is important for the discovery of the underlying biological mechanism, but don't forget proteomics is not just 2DE/2DLC coupled with MS identification, it can go much far beyond that. Proteomics for PTM, interactomics, proteomics target on specific organelle or protein complex, membrane proteomics, even topology for single protein, they all add more information for unveiling the biological mechanisms.....so my point is, if you want to do something 'deep' base on proteomics, you can
1 use conventional methods to validate your hypotheses derived from you proteomics data or
2 use new or more 'targeted' proteomics techniques to investigate the proteome further
3, if you are an engineer, develop a new proteomics workflow for a specific type of biological question/sample作者: 66小飞侠 时间: 2013-5-11 17:04
I agree with remenb,many people think proteomcis is just 2-DE+MS/MS, which may explain why we are discussing the question here. protein as the major function executor in a cell, It is the most important step to explain how gene regulate or determine phenotype.作者: 33号 时间: 2013-5-11 17:04
Well, if trained well, an undergraduate or a technician can do 2DE or 2DLC quite well just like you can, but what's essential is the data mining, hypotheses establishment and validation, from which you draw important biological conclusions, and that's what a Ph.D or postdoc about.作者: NBA 时间: 2013-5-11 17:06
蛋白质组的东西要做的深,一定要做功能。没有功能上不去。
Proteomic characterization of the human centrosome by protein correlation profiling, cuturl('www.ncbi.nlm.nih.gov/pubmed/14654843'),这篇文章其实他们01年就做完蛋白质组了,说是鉴定了500多个疑似Centrosome蛋白质,开会的时候我老板就问了一个问题:有多少个确实是centrosomal proteins? 结果这帮人就没话了。后来一方面是通过生物信息学作了个简单的算法,然后通过实验验证了十几个蛋白质。这才上的Nature。
总结就是,多想想:Where is your question? 你的技术也好,方法也好,仪器也要,要围绕你的question来转,而不是反之。OK,怎么找到你的question?非常简单,第一步,别人用某方法做A,你就用该方法做B;第二步,建立在第一步的基础上,改进改进方法,再去做C。作者: jude 时间: 2013-5-11 17:22
总结就是,多想想:Where is your question? 你的技术也好,方法也好,仪器也要,要围绕你的question来转,而不是反之。OK,怎么找到你的question?非常简单,第一步,别人用某方法做A,你就用该方法做B;第二步,建立在第一步的基础上,改进改进方法,再去做C。作者: plaa 时间: 2013-5-11 17:25