Trypsin inhibitor
from Glycine max (soybean)
Product Number T 9008
Storage Temperature 2-8 °C
Product Description
CAS Number: 9035-81-8
Extinction Coefficient: E1% = 9.94
(280 nm, pH 7.6 buffer)
pI: 4.51
Synonyms: Kunitz Trypsin Inhibitor, Tia1, STI, and
SBT1
This product is a 1% solution of trypsin in water.
Soybean trypsin inhibitor was first isolated by Kunitz.2
Several other related inhibitors are also found in
soybeans.3 Trypsin inhibitor from soybeans is a
monomeric protein containing 181 amino acid residues
in a single polypeptide chain crosslinked by two
disulfide bridges.4,5,6 The molecular weight
determined from the amino acid sequence is 20.1 kDa.
Soybean trypsin inhibitor inhibits trypsin, and to a
lesser extent chymotrypsin8 and plasmin.9 Soybean
trypsin inhibitor will also inhibit proteases with
mechanisms similar to trypsin. This product will also
inhibit plasma kallikrein and coagulation Factor XA.
However, this product will not inhibit metalloproteases,
tissue-based kallikrein, acid proteases, or thio
proteases.
Soybean trypsin inhibitor forms a 1:1 stoichiometric
complex with the protease active site. Upon formation
of this complex, trypsin may cleave a single arginineisoleucine
bond on the inhibitor.10,11 Inhibition is both
reversible and pH dependent. Dissociation of this
complex may yield a modified or native form of the
inhibitor.12 The optimal pH for trypsin binding is 8.0
with an association constant of greater than 109 at
pH 8.0 and 0.15 - 2.6 x 104 at pH 3.6 to 4.4.13
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以上是在SIGMA 网站上找到的信息，没有看到关于TYPE I II的介绍。可否告知链接？或是英文全名？也许以此做关键词可以找到。作者: wiwi 时间: 2013-7-3 16:26
只知道受体有TYPE I 和TYPE II 之分。作者: wiwi 时间: 2013-7-3 16:27
cuturl('http://www.sigmaaldrich.com/cgi-bin/hsrun/Distributed/HahtShop/HAHTpage/frmCatalogSearchPost?Brand=SIGMA&ProdNo=T6522')
Synonyms: SBTI
MDL number: MFCD00082103
CAS Number: 9035-81-8
MDL Number: MFCD00082103
Quality/Application: cell culture, tested
EC Number: 2329069
Storage Temp: 2-8°C
Miscellaneous: One trypsin unit = DA253 of 0.001 per minute with N-a-benzoyl-L-arginine ethyl ester (BAEE) as substrate at pH 7.6 at 25 °C. Reaction volume = 3.2 ml (1 cm light path).
Comments:
Type I-S, powder, cell culture, tested
Activity: (One mg will inhibit 1-3 mg of trypin with activity of approx. 10,000 BAEE units per mg protein.)
cuturl('http://www.sigmaaldrich.com/cgi-bin/hsrun/Distributed/HahtShop/HAHTpage/frmCatalogSearchPost?Brand=SIGMA&ProdNo=T9128')
Synonyms: SBTI
MDL number: MFCD00082103
CAS Number: 9035-81-8
MDL Number: MFCD00082103
EC Number: 2329069
Storage Temp: 2-8°C
Miscellaneous: One trypsin unit will produce a DA253 of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 ml, 1cm light path.
Comments:
Type II-S, Soluble powder
Unit definition: One trypsin unit will produce a DA253 of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 ml, 1cm light path.
One mg will inhibit minimum 1.0 mg of trypsin with activity of approx. 10,000 BAEE units per mg protein.作者: zzzz 时间: 2013-7-3 16:27
Thank you for contacting Sigma-Aldrich Technical Service. The type designations for these two product are Sigma in-house designations and do not represent any kind of biochemical significance. They are just used to differentiate between the two products. T9128 is a crude preparation of trypsin inhibitor (1 mg will inhibit 1 mg of trypsin with an activity of 10,000 BAEE units/mg protein). T6552 is of higher purity (1 mg will inhibit 1.0-3.0 mg ot trypsin with an activity of 10,000 BAEE units/mg protein), but more importantly, it has been suitability tested for use in cell culture applications (inhibition of trypsin after cells have been trypsinized). Thank you for your inquiry.
Regards,
Tom Glendening, Ph.D.,
Sigma-Aldrich Technical Service 作者: 3N4G 时间: 2013-7-3 16:28
