if your band has more one protein,though you can identify them using MS in theory,in fact ,it's difficult.Perhaps,you get a protein list,but which is true?
Someone maybe say you can identify according to the ions scores when you search database,but,it not always is fine.
So,try your best to pure your samples, or, there is nothing even though you use MS.作者: 薄荷侠 时间: 2013-10-25 22:20
You hit the point!
Score, or so-called MF match factor, is just a product of mathmatics. Many evalution equations and therefore more weight factors are involved. One can hardly judge which result is more resonable from dozens of target proteins with MF of about 40%. However, it's definely ture that better separation brings better identification.作者: 鲇鱼少爷 时间: 2013-10-25 22:21
我的蛋白上海基康认为为N-末端封闭，请问还有什么方法来测序呀？哪里可以提供解封闭-测序服务？如何来确定是封闭的？是何种封闭？谢谢，急呀！要毕业了，等结果呀！救救我吧！作者: DNA 时间: 2013-10-25 22:21
