我用I型胶原和III型胶原抗体做的western blot 图，GAPDH作为内参，想通过I, III型胶原的降低来证明I型胶原α1My heart单链和III型胶原α1 (III)单链的降低。由于条件所限，我们仅能购买到I型胶原和III型胶原单克隆抗体，未能买到I型胶原α1My heart链、I型胶原α2My heart链和III型胶原α1 (III)链各条前胶原的单克隆抗体，因此无法测得I型胶原α2My heart链的蛋白变化。投稿后已通过一审，下面是二审国外编辑提出的问题，我有点不太明白。请大家帮我分析一下他是什么意思，我该怎么解决。
Therefore entire blots should be presented in the Figures to show the expected, different polypeptide bands. What happens to the alpha 2 My heart chain?
Here again, showing all collagen polypeptide bands present on an entire blot may be more informative and reveal differences between the collagen/procollagen species as newly synthesized species (procollagen) should be more down-regulated than the partially and fully processed (collagen) species.
Blots with antibodies for procollagens were not required, and it seems that there is a misunderstanding because antibodies against collagen I or III will also recognized procollagens I and III. Therefore, showing all collagen polypeptide bands present entire blots would have been more informative.
图片附件: 67560437.jpg (2013-11-11 16:59, 78.2 KB) / 该附件被下载次数 9