分析测试百科

标题: 【求助】请教怎么看这份质谱报告？ [打印本页]

作者: misswu61 时间: 2014-3-27 23:18 标题: 【求助】请教怎么看这份质谱报告？

Mascot Search Results
User : wsf
Email : wusf@hupo.org.cn
Search title : Project: zxw, Spot Set: zxw\20071030-henduoren, Label: G2, Spot Id: 215653, Peak List Id: 101561, MS Job Run Id: 15911
MS data file : D:\zxw\1\li\ppw_G2_119379962924.txt
Database : NCBI_2007-3-16 0703 (4736044 sequences; 1634373987 residues)
Taxonomy : Mus musculus (house mouse) (104194 sequences)
Timestamp : 31 Oct 2007 at 14:34:40 GMT
Warning : A Peptide summary report will usually give a much clearer picture of MS/MS search results.
Top Score : 387 for gi|387134, alpha-A-crystallin
Probability Based Mowse Score
Ions score is -10*Log(P), where P is the probability that the observed match is a random event.
Protein scores greater than 63 are significant (p<0.05).
Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.
Protein Summary Report
Protein Summary (deprecated) Peptide Summary Select Summary (protein hits) Select Summary (unassigned) Export Search Results Help
Significance threshold p< Max. number of hits
Standard scoring MudPIT scoring Ions score cut-off Show sub-sets
Show pop-ups Suppress pop-ups Sort unassigned Decreasing Score Increasing query / Mr Decreasing Intensity Require bold red
Index
Accession Mass Score Description
1. gi|387134 18525 387 alpha-A-crystallin
2. gi|19526477 19780 381 crystallin, alpha A [Rattus norvegicus]
3. gi|30794510 22475 293 crystallin, alpha A [Mus musculus]
4. gi|553897 12198 227 alpha-A crystallin
5. gi|553898 14894 137 alpha-A-ins crystallin
6. gi|109541 15022 135 alpha-crystallin chain A, minor component - mouse (fragment)
7. gi|5777588 8280 133 alpha-crystallin [Mus musculus]
8. gi|41322925 516152 57 plectin 1 isoform 2 [Mus musculus]
9. gi|41322921 514572 54 plectin 1 isoform 3 [Mus musculus]
10. gi|41322927 506145 51 plectin 1 isoform 4 [Mus musculus]
11. gi|41322941 516999 51 plectin 1 isoform 11 [Mus musculus]
12. gi|41322935 517012 51 plectin 1 isoform 8 [Mus musculus]
13. gi|41322933 513447 51 plectin 1 isoform 7 [Mus musculus]
14. gi|94382128 48269 51 PREDICTED: similar to gonadotropin inducible ovarian transcription factor 1 [Mus musculus]
15. gi|17390900 55349 51 Ina protein [Mus musculus]
16. gi|5777589 10975 50 variant alpha-crystallin [Mus musculus]
17. gi|41322904 519466 48 plectin 1 isoform 1 [Mus musculus]
18. gi|41322939 517809 48 plectin 1 isoform 10 [Mus musculus]
19. gi|94371894 67614 46 PREDICTED: similar to zinc finger protein 618 [Mus musculus]
20. gi|74152994 39674 46 unnamed protein product [Mus musculus]

作者: misswu61 时间: 2014-3-27 23:18

Results List
1. gi|387134 Mass: 18525 Score: 387 Expect: 2.1e-034 Queries matched: 15
alpha-A-crystallin
Observed Mr(expt) Mr(calc) Delta Start End Miss Ions Peptide
744.39 743.38 743.38 -0.00 103 - 107 1 --- R.EFHRR.Y
980.58 979.58 979.57 0.00 61 - 68 0 53 K.FVIFLDVK.H
980.58 979.58 979.57 0.00 61 - 68 0 --- K.FVIFLDVK.H
1007.54 1006.54 1006.52 0.01 3 - 11 0 44 R.ALGPFYPSR.L
1007.54 1006.54 1006.52 0.01 3 - 11 0 --- R.ALGPFYPSR.L
1018.55 1017.54 1017.46 0.08 154 - 163 0 --- R.EEKPSSAPSS.-
1090.50 1089.49 1089.48 0.01 94 - 102 0 49 R.QDDHGYISR.E
1090.50 1089.49 1089.48 0.01 94 - 102 0 --- R.QDDHGYISR.E
1172.61 1171.61 1171.59 0.02 69 - 78 0 --- K.HFSPEDLTVK.V
1175.64 1174.63 1174.62 0.01 45 - 55 0 72 R.TVLDSGISEVR.S
1175.64 1174.63 1174.62 0.01 45 - 55 0 --- R.TVLDSGISEVR.S
1223.72 1222.72 1222.70 0.02 59 - 68 1 --- R.DKFVIFLDVK.H
1255.62 1254.61 1254.60 0.02 136 - 147 0 73 K.VQSGLDAGHSER.A
1255.62 1254.61 1254.60 0.02 136 - 147 0 --- K.VQSGLDAGHSER.A
1285.71 1284.70 1284.67 0.03 79 - 89 0 --- K.VLEDFVEIHGK.H
No match to: 728.15, 766.38, 822.38, 842.51, 870.54, 877.04, 961.53, 989.53, 990.52, 1002.57, 1005.52, 1006.51, 1019.54, 1021.52, 1023.54, 1029.52, 1045.51, 1051.51, 1072.49, 1073.47, 1112.48, 1157.62, 1179.62, 1184.60, 1186.62, 1191.59, 1194.59, 1197.62, 1206.56, 1207.57, 1209.59, 1237.61, 1277.61, 1307.69, 1323.66, 1398.70, 1430.69, 1442.71, 1443.75, 1457.72, 1459.75, 1462.76, 1465.73, 1473.72, 1475.75, 1481.73, 1491.74, 1497.72, 1581.90, 2230.24, 2638.38, 2682.40, 2698.42
2. gi|19526477 Mass: 19780 Score: 381 Expect: 8.3e-034 Queries matched: 15
crystallin, alpha A [Rattus norvegicus]
Observed Mr(expt) Mr(calc) Delta Start End Miss Ions Peptide
744.39 743.38 743.38 -0.00 113 - 117 1 --- R.EFHRR.Y
980.58 979.58 979.57 0.00 71 - 78 0 53 K.FVIFLDVK.H
980.58 979.58 979.57 0.00 71 - 78 0 --- K.FVIFLDVK.H
1007.54 1006.54 1006.52 0.01 13 - 21 0 44 R.ALGPFYPSR.L
1007.54 1006.54 1006.52 0.01 13 - 21 0 --- R.ALGPFYPSR.L
1018.55 1017.54 1017.46 0.08 164 - 173 0 --- R.EEKPSSAPSS.-
1090.50 1089.49 1089.48 0.01 104 - 112 0 49 R.QDDHGYISR.E
1090.50 1089.49 1089.48 0.01 104 - 112 0 --- R.QDDHGYISR.E
1172.61 1171.61 1171.59 0.02 79 - 88 0 --- K.HFSPEDLTVK.V
1175.64 1174.63 1174.62 0.01 55 - 65 0 72 R.TVLDSGISEVR.S
1175.64 1174.63 1174.62 0.01 55 - 65 0 --- R.TVLDSGISEVR.S
1223.72 1222.72 1222.70 0.02 69 - 78 1 --- R.DKFVIFLDVK.H
1255.62 1254.61 1254.60 0.02 146 - 157 0 73 K.VQSGLDAGHSER.A
1255.62 1254.61 1254.60 0.02 146 - 157 0 --- K.VQSGLDAGHSER.A
1285.71 1284.70 1284.67 0.03 89 - 99 0 --- K.VLEDFVEIHGK.H
No match to: 728.15, 766.38, 822.38, 842.51, 870.54, 877.04, 961.53, 989.53, 990.52, 1002.57, 1005.52, 1006.51, 1019.54, 1021.52, 1023.54, 1029.52, 1045.51, 1051.51, 1072.49, 1073.47, 1112.48, 1157.62, 1179.62, 1184.60, 1186.62, 1191.59, 1194.59, 1197.62, 1206.56, 1207.57, 1209.59, 1237.61, 1277.61, 1307.69, 1323.66, 1398.70, 1430.69, 1442.71, 1443.75, 1457.72, 1459.75, 1462.76, 1465.73, 1473.72, 1475.75, 1481.73, 1491.74, 1497.72, 1581.90, 2230.24, 2638.38, 2682.40, 2698.42
3. gi|30794510 Mass: 22475 Score: 293 Expect: 5.2e-025 Queries matched: 13
crystallin, alpha A [Mus musculus]

作者: misswu61 时间: 2014-3-27 23:19

Observed Mr(expt) Mr(calc) Delta Start End Miss Ions Peptide
744.39 743.38 743.38 -0.00 136 - 140 1 --- R.EFHRR.Y
980.58 979.58 979.57 0.00 94 - 101 0 53 K.FVIFLDVK.H
980.58 979.58 979.57 0.00 94 - 101 0 --- K.FVIFLDVK.H
1007.54 1006.54 1006.52 0.01 13 - 21 0 44 R.ALGPFYPSR.L
1007.54 1006.54 1006.52 0.01 13 - 21 0 --- R.ALGPFYPSR.L
1018.55 1017.54 1017.46 0.08 187 - 196 0 --- R.EEKPSSAPSS.-
1090.50 1089.49 1089.48 0.01 127 - 135 0 49 R.QDDHGYISR.E
1090.50 1089.49 1089.48 0.01 127 - 135 0 --- R.QDDHGYISR.E
1172.61 1171.61 1171.59 0.02 102 - 111 0 --- K.HFSPEDLTVK.V
1223.72 1222.72 1222.70 0.02 92 - 101 1 --- R.DKFVIFLDVK.H
1255.62 1254.61 1254.60 0.02 169 - 180 0 73 K.VQSGLDAGHSER.A
1255.62 1254.61 1254.60 0.02 169 - 180 0 --- K.VQSGLDAGHSER.A
1285.71 1284.70 1284.67 0.03 112 - 122 0 --- K.VLEDFVEIHGK.H
No match to: 728.15, 766.38, 822.38, 842.51, 870.54, 877.04, 961.53, 989.53, 990.52, 1002.57, 1005.52, 1006.51, 1019.54, 1021.52, 1023.54, 1029.52, 1045.51, 1051.51, 1072.49, 1073.47, 1112.48, 1157.62, 1175.64, 1175.64, 1179.62, 1184.60, 1186.62, 1191.59, 1194.59, 1197.62, 1206.56, 1207.57, 1209.59, 1237.61, 1277.61, 1307.69, 1323.66, 1398.70, 1430.69, 1442.71, 1443.75, 1457.72, 1459.75, 1462.76, 1465.73, 1473.72, 1475.75, 1481.73, 1491.74, 1497.72, 1581.90, 2230.24, 2638.38, 2682.40, 2698.42
4. gi|553897 Mass: 12198 Score: 227 Expect: 2.1e-018 Queries matched: 9
alpha-A crystallin
Observed Mr(expt) Mr(calc) Delta Start End Miss Ions Peptide
980.58 979.58 979.57 0.00 71 - 78 0 53 K.FVIFLDVK.H
980.58 979.58 979.57 0.00 71 - 78 0 --- K.FVIFLDVK.H
1007.54 1006.54 1006.52 0.01 13 - 21 0 44 R.ALGPFYPSR.L
1007.54 1006.54 1006.52 0.01 13 - 21 0 --- R.ALGPFYPSR.L
1172.61 1171.61 1171.59 0.02 79 - 88 0 --- K.HFSPEDLTVK.V
1175.64 1174.63 1174.62 0.01 55 - 65 0 72 R.TVLDSGISEVR.S
1175.64 1174.63 1174.62 0.01 55 - 65 0 --- R.TVLDSGISEVR.S
1223.72 1222.72 1222.70 0.02 69 - 78 1 --- R.DKFVIFLDVK.H
1285.71 1284.70 1284.67 0.03 89 - 99 0 --- K.VLEDFVEIHGK.H
No match to: 728.15, 744.39, 766.38, 822.38, 842.51, 870.54, 877.04, 961.53, 989.53, 990.52, 1002.57, 1005.52, 1006.51, 1018.55, 1019.54, 1021.52, 1023.54, 1029.52, 1045.51, 1051.51, 1072.49, 1073.47, 1090.50, 1090.50, 1112.48, 1157.62, 1179.62, 1184.60, 1186.62, 1191.59, 1194.59, 1197.62, 1206.56, 1207.57, 1209.59, 1237.61, 1255.62, 1255.62, 1277.61, 1307.69, 1323.66, 1398.70, 1430.69, 1442.71, 1443.75, 1457.72, 1459.75, 1462.76, 1465.73, 1473.72, 1475.75, 1481.73, 1491.74, 1497.72, 1581.90, 2230.24, 2638.38, 2682.40, 2698.42
5. gi|553898 Mass: 14894 Score: 137 Expect: 2.1e-009 Queries matched: 7
alpha-A-ins crystallin
Observed Mr(expt) Mr(calc) Delta Start End Miss Ions Peptide
980.58 979.58 979.57 0.00 94 - 101 0 53 K.FVIFLDVK.H
980.58 979.58 979.57 0.00 94 - 101 0 --- K.FVIFLDVK.H
1007.54 1006.54 1006.52 0.01 13 - 21 0 44 R.ALGPFYPSR.L
1007.54 1006.54 1006.52 0.01 13 - 21 0 --- R.ALGPFYPSR.L
1172.61 1171.61 1171.59 0.02 102 - 111 0 --- K.HFSPEDLTVK.V
1223.72 1222.72 1222.70 0.02 92 - 101 1 --- R.DKFVIFLDVK.H
1285.71 1284.70 1284.67 0.03 112 - 122 0 --- K.VLEDFVEIHGK.H
No match to: 728.15, 744.39, 766.38, 822.38, 842.51, 870.54, 877.04, 961.53, 989.53, 990.52, 1002.57, 1005.52, 1006.51, 1018.55, 1019.54, 1021.52, 1023.54, 1029.52, 1045.51, 1051.51, 1072.49, 1073.47, 1090.50, 1090.50, 1112.48, 1157.62, 1175.64, 1175.64, 1179.62, 1184.60, 1186.62, 1191.59, 1194.59, 1197.62, 1206.56, 1207.57, 1209.59, 1237.61, 1255.62, 1255.62, 1277.61, 1307.69, 1323.66, 1398.70, 1430.69, 1442.71, 1443.75, 1457.72, 1459.75, 1462.76, 1465.73, 1473.72, 1475.75, 1481.73, 1491.74, 1497.72, 1581.90, 2230.24, 2638.38, 2682.40, 2698.42
6. gi|109541 Mass: 15022 Score: 135 Expect: 3.3e-009 Queries matched: 7

作者: misswu61 时间: 2014-3-27 23:19

alpha-crystallin chain A, minor component - mouse (fragment)
Observed Mr(expt) Mr(calc) Delta Start End Miss Ions Peptide
980.58 979.58 979.57 0.00 94 - 101 0 53 K.FVIFLDVK.H
980.58 979.58 979.57 0.00 94 - 101 0 --- K.FVIFLDVK.H
1007.54 1006.54 1006.52 0.01 13 - 21 0 44 R.ALGPFYPSR.L
1007.54 1006.54 1006.52 0.01 13 - 21 0 --- R.ALGPFYPSR.L
1172.61 1171.61 1171.59 0.02 102 - 111 0 --- K.HFSPEDLTVK.V
1223.72 1222.72 1222.70 0.02 92 - 101 1 --- R.DKFVIFLDVK.H
1285.71 1284.70 1284.67 0.03 112 - 122 0 --- K.VLEDFVEIHGK.H
No match to: 728.15, 744.39, 766.38, 822.38, 842.51, 870.54, 877.04, 961.53, 989.53, 990.52, 1002.57, 1005.52, 1006.51, 1018.55, 1019.54, 1021.52, 1023.54, 1029.52, 1045.51, 1051.51, 1072.49, 1073.47, 1090.50, 1090.50, 1112.48, 1157.62, 1175.64, 1175.64, 1179.62, 1184.60, 1186.62, 1191.59, 1194.59, 1197.62, 1206.56, 1207.57, 1209.59, 1237.61, 1255.62, 1255.62, 1277.61, 1307.69, 1323.66, 1398.70, 1430.69, 1442.71, 1443.75, 1457.72, 1459.75, 1462.76, 1465.73, 1473.72, 1475.75, 1481.73, 1491.74, 1497.72, 1581.90, 2230.24, 2638.38, 2682.40, 2698.42
7. gi|5777588 Mass: 8280 Score: 133 Expect: 5.2e-009 Queries matched: 4
alpha-crystallin [Mus musculus]
Observed Mr(expt) Mr(calc) Delta Start End Miss Ions Peptide
1007.54 1006.54 1006.52 0.01 13 - 21 0 44 R.ALGPFYPSR.L
1007.54 1006.54 1006.52 0.01 13 - 21 0 --- R.ALGPFYPSR.L
1175.64 1174.63 1174.62 0.01 55 - 65 0 72 R.TVLDSGISEVR.S
1175.64 1174.63 1174.62 0.01 55 - 65 0 --- R.TVLDSGISEVR.S
No match to: 728.15, 744.39, 766.38, 822.38, 842.51, 870.54, 877.04, 961.53, 980.58, 980.58, 989.53, 990.52, 1002.57, 1005.52, 1006.51, 1018.55, 1019.54, 1021.52, 1023.54, 1029.52, 1045.51, 1051.51, 1072.49, 1073.47, 1090.50, 1090.50, 1112.48, 1157.62, 1172.61, 1179.62, 1184.60, 1186.62, 1191.59, 1194.59, 1197.62, 1206.56, 1207.57, 1209.59, 1223.72, 1237.61, 1255.62, 1255.62, 1277.61, 1285.71, 1307.69, 1323.66, 1398.70, 1430.69, 1442.71, 1443.75, 1457.72, 1459.75, 1462.76, 1465.73, 1473.72, 1475.75, 1481.73, 1491.74, 1497.72, 1581.90, 2230.24, 2638.38, 2682.40, 2698.42
8. gi|41322925 Mass: 516152 Score: 57 Expect: 0.22 Queries matched: 40
plectin 1 isoform 2 [Mus musculus]
Observed Mr(expt) Mr(calc) Delta Start End Miss Ions Peptide
744.39 743.38 743.39 -0.01 1338 - 1343 0 --- R.LAEQQR.A
766.38 765.37 765.39 -0.02 1768 - 1774 1 --- R.STSEKSK.Q
822.38 821.37 821.32 0.05 1332 - 1337 0 --- R.MEEEER.L
961.53 960.52 960.53 -0.01 3226 - 3234 0 --- K.VEVPVGSFK.G
989.53 988.52 988.52 0.00 1447 - 1454 0 --- R.LQLETTER.Q
990.52 989.52 989.50 0.01 1561 - 1569 0 --- R.SAEVELQSK.R
1002.57 1001.56 1001.53 0.04 2355 - 2362 1 --- R.TLEAERQR.Q
1007.54 1006.54 1006.54 -0.01 1920 - 1929 1 --- K.VSFEKAAAGK.A
1007.54 1006.54 1006.54 -0.01 1920 - 1929 1 --- K.VSFEKAAAGK.A
1018.55 1017.54 1017.50 0.04 1385 - 1392 1 --- R.MQEEVARR.E
1021.52 1020.52 1020.52 -0.00 2375 - 2382 1 --- K.LRMVEMSR.A
1023.54 1022.53 1022.54 -0.01 1151 - 1158 1 --- R.LQQRHGER.D
1029.52 1028.52 1028.54 -0.02 1775 - 1783 1 --- K.QRLEAEAGR.F
1045.51 1044.50 1044.48 0.02 1002 - 1010 1 --- K.DPARECAQR.I
1072.49 1071.48 1071.58 -0.10 1350 - 1359 0 --- R.LAEVEAALEK.Q
1073.47 1072.47 1072.51 -0.05 1962 - 1970 0 --- R.QLAAEEEQR.R
1090.50 1089.49 1089.53 -0.04 1415 - 1424 0 --- R.QSSEAEIQAK.A
1090.50 1089.49 1089.52 -0.03 2829 - 2838 0 --- R.ILSDPSDDTK.G
1157.62 1156.61 1156.61 0.00 2315 - 2324 0 --- K.AEAELLQQQK.E
1172.61 1171.61 1171.67 -0.07 153 - 161 1 --- K.EKLLLWSQR.M
1175.64 1174.63 1174.52 0.11 1708 - 1717 0 7 R.AETEQGEQQR.Q
1175.64 1174.63 1174.52 0.11 1708 - 1717 0 --- R.AETEQGEQQR.Q
1179.62 1178.62 1178.65 -0.03 2782 - 2791 1 --- K.DLIVRDHGVR.L
1186.62 1185.61 1185.64 -0.02 1738 - 1747 1 --- K.RQELEAELAK.V
1191.59 1190.58 1190.60 -0.03 1784 - 1793 1 --- R.FRELAEEAAR.L
1194.59 1193.59 1193.56 0.02 2377 - 2386 1 --- R.MVEMSRAQAR.A + Oxidation (M)
1197.62 1196.61 1196.61 -0.01 1727 - 1737 0 --- R.LQHEATAATQK.R
1209.59 1208.58 1208.63 -0.05 2586 - 2594 1 --- R.LQRLEEEHR.A
1255.62 1254.61 1254.63 -0.01 3354 - 3363 1 --- K.EKVTIYEAMR.R + Oxidation (M)
1255.62 1254.61 1254.63 -0.01 3354 - 3363 1 --- K.EKVTIYEAMR.R + Oxidation (M)
1285.71 1284.70 1284.70 -0.00 1739 - 1749 1 --- R.QELEAELAKVR.A
1307.69 1306.68 1306.72 -0.04 3991 - 4002 1 --- K.LLSAERAVTGYK.D
1323.66 1322.65 1322.72 -0.07 2945 - 2955 0 --- K.IVITVVEEHER.K
1398.70 1397.69 1397.79 -0.10 2313 - 2324 1 --- R.LKAEAELLQQQK.E
1430.69 1429.69 1429.76 -0.07 602 - 615 0 --- R.SLESLHGFVAAATK.E
1443.75 1442.74 1442.75 -0.01 2996 - 3008 1 --- R.EVAEADSVRQALR.G
1457.72 1456.72 1456.62 0.10 171 - 182 1 --- R.CDNFTTSWRDGR.L
1473.72 1472.72 1472.66 0.06 452 - 463 1 --- K.DGRHPQGEQMYR.R
1481.73 1480.72 1480.72 0.01 4186 - 4198 1 --- R.SGRQYDIDDAITK.N
1497.72 1496.71 1496.61 0.11 624 - 635 0 --- K.EEEEVGFDWSDR.N
No match to: 728.15, 842.51, 870.54, 877.04, 980.58, 980.58, 1005.52, 1006.51, 1019.54, 1051.51, 1112.48, 1184.60, 1206.56, 1207.57, 1223.72, 1237.61, 1277.61, 1442.71, 1459.75, 1462.76, 1465.73, 1475.75, 1491.74, 1581.90, 2230.24, 2638.38, 2682.40, 2698.42
9. gi|41322921 Mass: 514572 Score: 54 Expect: 0.39 Queries matched: 40
plectin 1 isoform 3 [Mus musculus]

作者: misswu61 时间: 2014-3-27 23:19

Observed Mr(expt) Mr(calc) Delta Start End Miss Ions Peptide
744.39 743.38 743.39 -0.01 1325 - 1330 0 --- R.LAEQQR.A
766.38 765.37 765.39 -0.02 1755 - 1761 1 --- R.STSEKSK.Q
822.38 821.37 821.32 0.05 1319 - 1324 0 --- R.MEEEER.L
961.53 960.52 960.53 -0.01 3213 - 3221 0 --- K.VEVPVGSFK.G
989.53 988.52 988.52 0.00 1434 - 1441 0 --- R.LQLETTER.Q
990.52 989.52 989.50 0.01 1548 - 1556 0 --- R.SAEVELQSK.R
1002.57 1001.56 1001.53 0.04 2342 - 2349 1 --- R.TLEAERQR.Q
1007.54 1006.54 1006.54 -0.01 1907 - 1916 1 --- K.VSFEKAAAGK.A
1007.54 1006.54 1006.54 -0.01 1907 - 1916 1 --- K.VSFEKAAAGK.A
1018.55 1017.54 1017.50 0.04 1372 - 1379 1 --- R.MQEEVARR.E
1021.52 1020.52 1020.52 -0.00 2362 - 2369 1 --- K.LRMVEMSR.A
1023.54 1022.53 1022.54 -0.01 1138 - 1145 1 --- R.LQQRHGER.D
1029.52 1028.52 1028.54 -0.02 1762 - 1770 1 --- K.QRLEAEAGR.F
1045.51 1044.50 1044.48 0.02 989 - 997 1 --- K.DPARECAQR.I
1072.49 1071.48 1071.58 -0.10 1337 - 1346 0 --- R.LAEVEAALEK.Q
1073.47 1072.47 1072.51 -0.05 1949 - 1957 0 --- R.QLAAEEEQR.R
1090.50 1089.49 1089.53 -0.04 1402 - 1411 0 --- R.QSSEAEIQAK.A
1090.50 1089.49 1089.52 -0.03 2816 - 2825 0 --- R.ILSDPSDDTK.G
1157.62 1156.61 1156.61 0.00 2302 - 2311 0 --- K.AEAELLQQQK.E
1172.61 1171.61 1171.67 -0.07 140 - 148 1 --- K.EKLLLWSQR.M
1175.64 1174.63 1174.52 0.11 1695 - 1704 0 7 R.AETEQGEQQR.Q
1175.64 1174.63 1174.52 0.11 1695 - 1704 0 --- R.AETEQGEQQR.Q
1179.62 1178.62 1178.65 -0.03 2769 - 2778 1 --- K.DLIVRDHGVR.L
1186.62 1185.61 1185.64 -0.02 1725 - 1734 1 --- K.RQELEAELAK.V
1191.59 1190.58 1190.60 -0.03 1771 - 1780 1 --- R.FRELAEEAAR.L
1194.59 1193.59 1193.56 0.02 2364 - 2373 1 --- R.MVEMSRAQAR.A + Oxidation (M)
1197.62 1196.61 1196.61 -0.01 1714 - 1724 0 --- R.LQHEATAATQK.R
1209.59 1208.58 1208.63 -0.05 2573 - 2581 1 --- R.LQRLEEEHR.A
1255.62 1254.61 1254.63 -0.01 3341 - 3350 1 --- K.EKVTIYEAMR.R + Oxidation (M)
1255.62 1254.61 1254.63 -0.01 3341 - 3350 1 --- K.EKVTIYEAMR.R + Oxidation (M)
1285.71 1284.70 1284.70 -0.00 1726 - 1736 1 --- R.QELEAELAKVR.A
1307.69 1306.68 1306.72 -0.04 3978 - 3989 1 --- K.LLSAERAVTGYK.D
1323.66 1322.65 1322.72 -0.07 2932 - 2942 0 --- K.IVITVVEEHER.K
1398.70 1397.69 1397.79 -0.10 2300 - 2311 1 --- R.LKAEAELLQQQK.E
1430.69 1429.69 1429.76 -0.07 589 - 602 0 --- R.SLESLHGFVAAATK.E
1443.75 1442.74 1442.75 -0.01 2983 - 2995 1 --- R.EVAEADSVRQALR.G
1457.72 1456.72 1456.62 0.10 158 - 169 1 --- R.CDNFTTSWRDGR.L
1473.72 1472.72 1472.66 0.06 439 - 450 1 --- K.DGRHPQGEQMYR.R
1481.73 1480.72 1480.72 0.01 4173 - 4185 1 --- R.SGRQYDIDDAITK.N
1497.72 1496.71 1496.61 0.11 611 - 622 0 --- K.EEEEVGFDWSDR.N
No match to: 728.15, 842.51, 870.54, 877.04, 980.58, 980.58, 1005.52, 1006.51, 1019.54, 1051.51, 1112.48, 1184.60, 1206.56, 1207.57, 1223.72, 1237.61, 1277.61, 1442.71, 1459.75, 1462.76, 1465.73, 1475.75, 1491.74, 1581.90, 2230.24, 2638.38, 2682.40, 2698.42
10. gi|41322927 Mass: 506145 Score: 51 Expect: 0.75 Queries matched: 40
plectin 1 isoform 4 [Mus musculus]

作者: misswu61 时间: 2014-3-27 23:20

Observed Mr(expt) Mr(calc) Delta Start End Miss Ions Peptide
744.39 743.38 743.39 -0.01 1253 - 1258 0 --- R.LAEQQR.A
766.38 765.37 765.39 -0.02 1683 - 1689 1 --- R.STSEKSK.Q
822.38 821.37 821.32 0.05 1247 - 1252 0 --- R.MEEEER.L
961.53 960.52 960.53 -0.01 3141 - 3149 0 --- K.VEVPVGSFK.G
989.53 988.52 988.52 0.00 1362 - 1369 0 --- R.LQLETTER.Q
990.52 989.52 989.50 0.01 1476 - 1484 0 --- R.SAEVELQSK.R
1002.57 1001.56 1001.53 0.04 2270 - 2277 1 --- R.TLEAERQR.Q
1007.54 1006.54 1006.54 -0.01 1835 - 1844 1 --- K.VSFEKAAAGK.A
1007.54 1006.54 1006.54 -0.01 1835 - 1844 1 --- K.VSFEKAAAGK.A
1018.55 1017.54 1017.50 0.04 1300 - 1307 1 --- R.MQEEVARR.E
1021.52 1020.52 1020.52 -0.00 2290 - 2297 1 --- K.LRMVEMSR.A
1023.54 1022.53 1022.54 -0.01 1066 - 1073 1 --- R.LQQRHGER.D
1029.52 1028.52 1028.54 -0.02 1690 - 1698 1 --- K.QRLEAEAGR.F
1045.51 1044.50 1044.48 0.02 917 - 925 1 --- K.DPARECAQR.I
1072.49 1071.48 1071.58 -0.10 1265 - 1274 0 --- R.LAEVEAALEK.Q
1073.47 1072.47 1072.51 -0.05 1877 - 1885 0 --- R.QLAAEEEQR.R
1090.50 1089.49 1089.53 -0.04 1330 - 1339 0 --- R.QSSEAEIQAK.A
1090.50 1089.49 1089.52 -0.03 2744 - 2753 0 --- R.ILSDPSDDTK.G
1157.62 1156.61 1156.61 0.00 2230 - 2239 0 --- K.AEAELLQQQK.E
1172.61 1171.61 1171.67 -0.07 68 - 76 1 --- K.EKLLLWSQR.M
1175.64 1174.63 1174.52 0.11 1623 - 1632 0 7 R.AETEQGEQQR.Q
1175.64 1174.63 1174.52 0.11 1623 - 1632 0 --- R.AETEQGEQQR.Q
1179.62 1178.62 1178.65 -0.03 2697 - 2706 1 --- K.DLIVRDHGVR.L
1186.62 1185.61 1185.64 -0.02 1653 - 1662 1 --- K.RQELEAELAK.V
1191.59 1190.58 1190.60 -0.03 1699 - 1708 1 --- R.FRELAEEAAR.L
1194.59 1193.59 1193.56 0.02 2292 - 2301 1 --- R.MVEMSRAQAR.A + Oxidation (M)
1197.62 1196.61 1196.61 -0.01 1642 - 1652 0 --- R.LQHEATAATQK.R
1209.59 1208.58 1208.63 -0.05 2501 - 2509 1 --- R.LQRLEEEHR.A
1255.62 1254.61 1254.63 -0.01 3269 - 3278 1 --- K.EKVTIYEAMR.R + Oxidation (M)
1255.62 1254.61 1254.63 -0.01 3269 - 3278 1 --- K.EKVTIYEAMR.R + Oxidation (M)
1285.71 1284.70 1284.70 -0.00 1654 - 1664 1 --- R.QELEAELAKVR.A
1307.69 1306.68 1306.72 -0.04 3906 - 3917 1 --- K.LLSAERAVTGYK.D
1323.66 1322.65 1322.72 -0.07 2860 - 2870 0 --- K.IVITVVEEHER.K
1398.70 1397.69 1397.79 -0.10 2228 - 2239 1 --- R.LKAEAELLQQQK.E
1430.69 1429.69 1429.76 -0.07 517 - 530 0 --- R.SLESLHGFVAAATK.E
1443.75 1442.74 1442.75 -0.01 2911 - 2923 1 --- R.EVAEADSVRQALR.G
1457.72 1456.72 1456.62 0.10 86 - 97 1 --- R.CDNFTTSWRDGR.L
1473.72 1472.72 1472.66 0.06 367 - 378 1 --- K.DGRHPQGEQMYR.R
1481.73 1480.72 1480.72 0.01 4101 - 4113 1 --- R.SGRQYDIDDAITK.N
1497.72 1496.71 1496.61 0.11 539 - 550 0 --- K.EEEEVGFDWSDR.N
No match to: 728.15, 842.51, 870.54, 877.04, 980.58, 980.58, 1005.52, 1006.51, 1019.54, 1051.51, 1112.48, 1184.60, 1206.56, 1207.57, 1223.72, 1237.61, 1277.61, 1442.71, 1459.75, 1462.76, 1465.73, 1475.75, 1491.74, 1581.90, 2230.24, 2638.38, 2682.40, 2698.42
11. gi|41322941 Mass: 516999 Score: 51 Expect: 0.81 Queries matched: 40
plectin 1 isoform 11 [Mus musculus]
Observed Mr(expt) Mr(calc) Delta Start End Miss Ions Peptide
744.39 743.38 743.39 -0.01 1347 - 1352 0 --- R.LAEQQR.A
766.38 765.37 765.39 -0.02 1777 - 1783 1 --- R.STSEKSK.Q
822.38 821.37 821.32 0.05 1341 - 1346 0 --- R.MEEEER.L
961.53 960.52 960.53 -0.01 3235 - 3243 0 --- K.VEVPVGSFK.G
989.53 988.52 988.52 0.00 1456 - 1463 0 --- R.LQLETTER.Q
990.52 989.52 989.50 0.01 1570 - 1578 0 --- R.SAEVELQSK.R
1002.57 1001.56 1001.53 0.04 2364 - 2371 1 --- R.TLEAERQR.Q
1007.54 1006.54 1006.54 -0.01 1929 - 1938 1 --- K.VSFEKAAAGK.A
1007.54 1006.54 1006.54 -0.01 1929 - 1938 1 --- K.VSFEKAAAGK.A
1018.55 1017.54 1017.50 0.04 1394 - 1401 1 --- R.MQEEVARR.E
1021.52 1020.52 1020.52 -0.00 2384 - 2391 1 --- K.LRMVEMSR.A
1023.54 1022.53 1022.54 -0.01 1160 - 1167 1 --- R.LQQRHGER.D
1029.52 1028.52 1028.54 -0.02 1784 - 1792 1 --- K.QRLEAEAGR.F
1045.51 1044.50 1044.48 0.02 1011 - 1019 1 --- K.DPARECAQR.I
1072.49 1071.48 1071.58 -0.10 1359 - 1368 0 --- R.LAEVEAALEK.Q
1073.47 1072.47 1072.51 -0.05 1971 - 1979 0 --- R.QLAAEEEQR.R
1090.50 1089.49 1089.53 -0.04 1424 - 1433 0 --- R.QSSEAEIQAK.A
1090.50 1089.49 1089.52 -0.03 2838 - 2847 0 --- R.ILSDPSDDTK.G
1157.62 1156.61 1156.61 0.00 2324 - 2333 0 --- K.AEAELLQQQK.E
1172.61 1171.61 1171.67 -0.07 162 - 170 1 --- K.EKLLLWSQR.M
1175.64 1174.63 1174.52 0.11 1717 - 1726 0 7 R.AETEQGEQQR.Q
1175.64 1174.63 1174.52 0.11 1717 - 1726 0 --- R.AETEQGEQQR.Q
1179.62 1178.62 1178.65 -0.03 2791 - 2800 1 --- K.DLIVRDHGVR.L
1186.62 1185.61 1185.64 -0.02 1747 - 1756 1 --- K.RQELEAELAK.V
1191.59 1190.58 1190.60 -0.03 1793 - 1802 1 --- R.FRELAEEAAR.L
1194.59 1193.59 1193.56 0.02 2386 - 2395 1 --- R.MVEMSRAQAR.A + Oxidation (M)
1197.62 1196.61 1196.61 -0.01 1736 - 1746 0 --- R.LQHEATAATQK.R
1209.59 1208.58 1208.63 -0.05 2595 - 2603 1 --- R.LQRLEEEHR.A
1255.62 1254.61 1254.63 -0.01 3363 - 3372 1 --- K.EKVTIYEAMR.R + Oxidation (M)
1255.62 1254.61 1254.63 -0.01 3363 - 3372 1 --- K.EKVTIYEAMR.R + Oxidation (M)
1285.71 1284.70 1284.70 -0.00 1748 - 1758 1 --- R.QELEAELAKVR.A
1307.69 1306.68 1306.72 -0.04 4000 - 4011 1 --- K.LLSAERAVTGYK.D
1323.66 1322.65 1322.72 -0.07 2954 - 2964 0 --- K.IVITVVEEHER.K
1398.70 1397.69 1397.79 -0.10 2322 - 2333 1 --- R.LKAEAELLQQQK.E
1430.69 1429.69 1429.76 -0.07 611 - 624 0 --- R.SLESLHGFVAAATK.E
1443.75 1442.74 1442.75 -0.01 3005 - 3017 1 --- R.EVAEADSVRQALR.G
1457.72 1456.72 1456.62 0.10 180 - 191 1 --- R.CDNFTTSWRDGR.L
1473.72 1472.72 1472.66 0.06 461 - 472 1 --- K.DGRHPQGEQMYR.R
1481.73 1480.72 1480.72 0.01 4195 - 4207 1 --- R.SGRQYDIDDAITK.N
1497.72 1496.71 1496.61 0.11 633 - 644 0 --- K.EEEEVGFDWSDR.N
No match to: 728.15, 842.51, 870.54, 877.04, 980.58, 980.58, 1005.52, 1006.51, 1019.54, 1051.51, 1112.48, 1184.60, 1206.56, 1207.57, 1223.72, 1237.61, 1277.61, 1442.71, 1459.75, 1462.76, 1465.73, 1475.75, 1491.74, 1581.90, 2230.24, 2638.38, 2682.40, 2698.42
12. gi|41322935 Mass: 517012 Score: 51 Expect: 0.81 Queries matched: 40
plectin 1 isoform 8 [Mus musculus]

作者: misswu61 时间: 2014-3-27 23:20

Observed Mr(expt) Mr(calc) Delta Start End Miss Ions Peptide
744.39 743.38 743.39 -0.01 1347 - 1352 0 --- R.LAEQQR.A
766.38 765.37 765.39 -0.02 1777 - 1783 1 --- R.STSEKSK.Q
822.38 821.37 821.32 0.05 1341 - 1346 0 --- R.MEEEER.L
961.53 960.52 960.53 -0.01 3235 - 3243 0 --- K.VEVPVGSFK.G
989.53 988.52 988.52 0.00 1456 - 1463 0 --- R.LQLETTER.Q
990.52 989.52 989.50 0.01 1570 - 1578 0 --- R.SAEVELQSK.R
1002.57 1001.56 1001.53 0.04 2364 - 2371 1 --- R.TLEAERQR.Q
1007.54 1006.54 1006.54 -0.01 1929 - 1938 1 --- K.VSFEKAAAGK.A
1007.54 1006.54 1006.54 -0.01 1929 - 1938 1 --- K.VSFEKAAAGK.A
1018.55 1017.54 1017.50 0.04 1394 - 1401 1 --- R.MQEEVARR.E
1021.52 1020.52 1020.52 -0.00 2384 - 2391 1 --- K.LRMVEMSR.A
1023.54 1022.53 1022.54 -0.01 1160 - 1167 1 --- R.LQQRHGER.D
1029.52 1028.52 1028.54 -0.02 1784 - 1792 1 --- K.QRLEAEAGR.F
1045.51 1044.50 1044.48 0.02 1011 - 1019 1 --- K.DPARECAQR.I
1072.49 1071.48 1071.58 -0.10 1359 - 1368 0 --- R.LAEVEAALEK.Q
1073.47 1072.47 1072.51 -0.05 1971 - 1979 0 --- R.QLAAEEEQR.R
1090.50 1089.49 1089.53 -0.04 1424 - 1433 0 --- R.QSSEAEIQAK.A
1090.50 1089.49 1089.52 -0.03 2838 - 2847 0 --- R.ILSDPSDDTK.G
1157.62 1156.61 1156.61 0.00 2324 - 2333 0 --- K.AEAELLQQQK.E
1172.61 1171.61 1171.67 -0.07 162 - 170 1 --- K.EKLLLWSQR.M
1175.64 1174.63 1174.52 0.11 1717 - 1726 0 7 R.AETEQGEQQR.Q
1175.64 1174.63 1174.52 0.11 1717 - 1726 0 --- R.AETEQGEQQR.Q
1179.62 1178.62 1178.65 -0.03 2791 - 2800 1 --- K.DLIVRDHGVR.L
1186.62 1185.61 1185.64 -0.02 1747 - 1756 1 --- K.RQELEAELAK.V
1191.59 1190.58 1190.60 -0.03 1793 - 1802 1 --- R.FRELAEEAAR.L
1194.59 1193.59 1193.56 0.02 2386 - 2395 1 --- R.MVEMSRAQAR.A + Oxidation (M)
1197.62 1196.61 1196.61 -0.01 1736 - 1746 0 --- R.LQHEATAATQK.R
1209.59 1208.58 1208.63 -0.05 2595 - 2603 1 --- R.LQRLEEEHR.A
1255.62 1254.61 1254.63 -0.01 3363 - 3372 1 --- K.EKVTIYEAMR.R + Oxidation (M)
1255.62 1254.61 1254.63 -0.01 3363 - 3372 1 --- K.EKVTIYEAMR.R + Oxidation (M)
1285.71 1284.70 1284.70 -0.00 1748 - 1758 1 --- R.QELEAELAKVR.A
1307.69 1306.68 1306.72 -0.04 4000 - 4011 1 --- K.LLSAERAVTGYK.D
1323.66 1322.65 1322.72 -0.07 2954 - 2964 0 --- K.IVITVVEEHER.K
1398.70 1397.69 1397.79 -0.10 2322 - 2333 1 --- R.LKAEAELLQQQK.E
1430.69 1429.69 1429.76 -0.07 611 - 624 0 --- R.SLESLHGFVAAATK.E
1443.75 1442.74 1442.75 -0.01 3005 - 3017 1 --- R.EVAEADSVRQALR.G
1457.72 1456.72 1456.62 0.10 180 - 191 1 --- R.CDNFTTSWRDGR.L
1473.72 1472.72 1472.66 0.06 461 - 472 1 --- K.DGRHPQGEQMYR.R
1481.73 1480.72 1480.72 0.01 4195 - 4207 1 --- R.SGRQYDIDDAITK.N
1497.72 1496.71 1496.61 0.11 633 - 644 0 --- K.EEEEVGFDWSDR.N
No match to: 728.15, 842.51, 870.54, 877.04, 980.58, 980.58, 1005.52, 1006.51, 1019.54, 1051.51, 1112.48, 1184.60, 1206.56, 1207.57, 1223.72, 1237.61, 1277.61, 1442.71, 1459.75, 1462.76, 1465.73, 1475.75, 1491.74, 1581.90, 2230.24, 2638.38, 2682.40, 2698.42
13. gi|41322933 Mass: 513447 Score: 51 Expect: 0.81 Queries matched: 40
plectin 1 isoform 7 [Mus musculus]

作者: misswu61 时间: 2014-3-27 23:20

Observed Mr(expt) Mr(calc) Delta Start End Miss Ions Peptide
744.39 743.38 743.39 -0.01 1315 - 1320 0 --- R.LAEQQR.A
766.38 765.37 765.39 -0.02 1745 - 1751 1 --- R.STSEKSK.Q
822.38 821.37 821.32 0.05 1309 - 1314 0 --- R.MEEEER.L
961.53 960.52 960.53 -0.01 3203 - 3211 0 --- K.VEVPVGSFK.G
989.53 988.52 988.52 0.00 1424 - 1431 0 --- R.LQLETTER.Q
990.52 989.52 989.50 0.01 1538 - 1546 0 --- R.SAEVELQSK.R
1002.57 1001.56 1001.53 0.04 2332 - 2339 1 --- R.TLEAERQR.Q
1007.54 1006.54 1006.54 -0.01 1897 - 1906 1 --- K.VSFEKAAAGK.A
1007.54 1006.54 1006.54 -0.01 1897 - 1906 1 --- K.VSFEKAAAGK.A
1018.55 1017.54 1017.50 0.04 1362 - 1369 1 --- R.MQEEVARR.E
1021.52 1020.52 1020.52 -0.00 2352 - 2359 1 --- K.LRMVEMSR.A
1023.54 1022.53 1022.54 -0.01 1128 - 1135 1 --- R.LQQRHGER.D
1029.52 1028.52 1028.54 -0.02 1752 - 1760 1 --- K.QRLEAEAGR.F
1045.51 1044.50 1044.48 0.02 979 - 987 1 --- K.DPARECAQR.I
1072.49 1071.48 1071.58 -0.10 1327 - 1336 0 --- R.LAEVEAALEK.Q
1073.47 1072.47 1072.51 -0.05 1939 - 1947 0 --- R.QLAAEEEQR.R
1090.50 1089.49 1089.53 -0.04 1392 - 1401 0 --- R.QSSEAEIQAK.A
1090.50 1089.49 1089.52 -0.03 2806 - 2815 0 --- R.ILSDPSDDTK.G
1157.62 1156.61 1156.61 0.00 2292 - 2301 0 --- K.AEAELLQQQK.E
1172.61 1171.61 1171.67 -0.07 130 - 138 1 --- K.EKLLLWSQR.M
1175.64 1174.63 1174.52 0.11 1685 - 1694 0 7 R.AETEQGEQQR.Q
1175.64 1174.63 1174.52 0.11 1685 - 1694 0 --- R.AETEQGEQQR.Q
1179.62 1178.62 1178.65 -0.03 2759 - 2768 1 --- K.DLIVRDHGVR.L
1186.62 1185.61 1185.64 -0.02 1715 - 1724 1 --- K.RQELEAELAK.V
1191.59 1190.58 1190.60 -0.03 1761 - 1770 1 --- R.FRELAEEAAR.L
1194.59 1193.59 1193.56 0.02 2354 - 2363 1 --- R.MVEMSRAQAR.A + Oxidation (M)
1197.62 1196.61 1196.61 -0.01 1704 - 1714 0 --- R.LQHEATAATQK.R
1209.59 1208.58 1208.63 -0.05 2563 - 2571 1 --- R.LQRLEEEHR.A
1255.62 1254.61 1254.63 -0.01 3331 - 3340 1 --- K.EKVTIYEAMR.R + Oxidation (M)
1255.62 1254.61 1254.63 -0.01 3331 - 3340 1 --- K.EKVTIYEAMR.R + Oxidation (M)
1285.71 1284.70 1284.70 -0.00 1716 - 1726 1 --- R.QELEAELAKVR.A
1307.69 1306.68 1306.72 -0.04 3968 - 3979 1 --- K.LLSAERAVTGYK.D
1323.66 1322.65 1322.72 -0.07 2922 - 2932 0 --- K.IVITVVEEHER.K
1398.70 1397.69 1397.79 -0.10 2290 - 2301 1 --- R.LKAEAELLQQQK.E
1430.69 1429.69 1429.76 -0.07 579 - 592 0 --- R.SLESLHGFVAAATK.E
1443.75 1442.74 1442.75 -0.01 2973 - 2985 1 --- R.EVAEADSVRQALR.G
1457.72 1456.72 1456.62 0.10 148 - 159 1 --- R.CDNFTTSWRDGR.L
1473.72 1472.72 1472.66 0.06 429 - 440 1 --- K.DGRHPQGEQMYR.R
1481.73 1480.72 1480.72 0.01 4163 - 4175 1 --- R.SGRQYDIDDAITK.N
1497.72 1496.71 1496.61 0.11 601 - 612 0 --- K.EEEEVGFDWSDR.N
No match to: 728.15, 842.51, 870.54, 877.04, 980.58, 980.58, 1005.52, 1006.51, 1019.54, 1051.51, 1112.48, 1184.60, 1206.56, 1207.57, 1223.72, 1237.61, 1277.61, 1442.71, 1459.75, 1462.76, 1465.73, 1475.75, 1491.74, 1581.90, 2230.24, 2638.38, 2682.40, 2698.42
14. gi|94382128 Mass: 48269 Score: 51 Expect: 0.85 Queries matched: 11
PREDICTED: similar to gonadotropin inducible ovarian transcription factor 1 [Mus musculus]
Observed Mr(expt) Mr(calc) Delta Start End Miss Ions Peptide

作者: misswu61 时间: 2014-3-27 23:20

870.54 869.54 869.45 0.08 401 - 408 1 --- K.VMHTGGRL.-
1002.57 1001.56 1001.50 0.06 255 - 261 1 --- K.YLREHER.T
1051.51 1050.50 1050.53 -0.03 124 - 132 0 --- R.TDTAQKPYK.C
1072.49 1071.48 1071.45 0.04 184 - 192 0 --- R.SYICNQCNK.A
1179.62 1178.62 1178.61 0.01 39 - 48 0 --- R.NLTAIGYNWK.D
1207.57 1206.56 1206.64 -0.08 123 - 132 1 --- K.RTDTAQKPYK.C
1398.70 1397.69 1397.74 -0.05 389 - 400 1 --- K.AFTQSTHLKTHK.V
1443.75 1442.74 1442.72 0.02 28 - 38 1 --- R.LYKDVMLENYR.N
1459.75 1458.74 1458.72 0.02 28 - 38 1 --- R.LYKDVMLENYR.N + Oxidation (M)
1473.72 1472.72 1472.81 -0.10 111 - 123 1 --- K.VLACQSTLQINKR.T
2638.38 2637.37 2637.21 0.17 333 - 354 1 --- K.AYSQQSCLQMHERTHTGEKPYK.C + Oxidation (M)
No match to: 728.15, 744.39, 766.38, 822.38, 842.51, 877.04, 961.53, 980.58, 980.58, 989.53, 990.52, 1005.52, 1006.51, 1007.54, 1007.54, 1018.55, 1019.54, 1021.52, 1023.54, 1029.52, 1045.51, 1073.47, 1090.50, 1090.50, 1112.48, 1157.62, 1172.61, 1175.64, 1175.64, 1184.60, 1186.62, 1191.59, 1194.59, 1197.62, 1206.56, 1209.59, 1223.72, 1237.61, 1255.62, 1255.62, 1277.61, 1285.71, 1307.69, 1323.66, 1430.69, 1442.71, 1457.72, 1462.76, 1465.73, 1475.75, 1481.73, 1491.74, 1497.72, 1581.90, 2230.24, 2682.40, 2698.42
15. gi|17390900 Mass: 55349 Score: 51 Expect: 0.89 Queries matched: 11
Ina protein [Mus musculus]
Observed Mr(expt) Mr(calc) Delta Start End Miss Ions Peptide
980.58 979.58 979.49 0.08 131 - 138 1 --- R.QRHAEPSR.V
980.58 979.58 979.50 0.08 270 - 278 0 --- R.AQYESLAAK.N
989.53 988.52 988.59 -0.07 439 - 448 1 --- K.VSSAGLSLKK.E
990.52 989.52 989.53 -0.01 301 - 309 1 --- R.STEAIRASR.E
1021.52 1020.52 1020.50 0.02 29 - 39 0 --- R.LSGPGGSGSFR.S
1029.52 1028.52 1028.55 -0.03 339 - 346 0 --- R.QILELEER.H
1072.49 1071.48 1071.53 -0.05 96 - 104 0 --- K.EQLQGLNDR.F
1194.59 1193.59 1193.62 -0.03 18 - 28 1 --- K.VFGDSSRLSAR.L
1285.71 1284.70 1284.68 0.02 169 - 179 1 --- R.DGLAEEVQRLR.A
1459.75 1458.74 1458.74 -0.00 149 - 161 1 --- R.ELRAQLEEASSAR.A
1465.73 1464.72 1464.61 0.11 448 - 459 1 --- K.KEEEEEEEEASK.E
No match to: 728.15, 744.39, 766.38, 822.38, 842.51, 870.54, 877.04, 961.53, 1002.57, 1005.52, 1006.51, 1007.54, 1007.54, 1018.55, 1019.54, 1023.54, 1045.51, 1051.51, 1073.47, 1090.50, 1090.50, 1112.48, 1157.62, 1172.61, 1175.64, 1175.64, 1179.62, 1184.60, 1186.62, 1191.59, 1197.62, 1206.56, 1207.57, 1209.59, 1223.72, 1237.61, 1255.62, 1255.62, 1277.61, 1307.69, 1323.66, 1398.70, 1430.69, 1442.71, 1443.75, 1457.72, 1462.76, 1473.72, 1475.75, 1481.73, 1491.74, 1497.72, 1581.90, 2230.24, 2638.38, 2682.40, 2698.42
16. gi|5777589 Mass: 10975 Score: 50 Expect: 0.97 Queries matched: 2
variant alpha-crystallin [Mus musculus]
Observed Mr(expt) Mr(calc) Delta Start End Miss Ions Peptide
1007.54 1006.54 1006.52 0.01 13 - 21 0 44 R.ALGPFYPSR.L
1007.54 1006.54 1006.52 0.01 13 - 21 0 --- R.ALGPFYPSR.L
No match to: 728.15, 744.39, 766.38, 822.38, 842.51, 870.54, 877.04, 961.53, 980.58, 980.58, 989.53, 990.52, 1002.57, 1005.52, 1006.51, 1018.55, 1019.54, 1021.52, 1023.54, 1029.52, 1045.51, 1051.51, 1072.49, 1073.47, 1090.50, 1090.50, 1112.48, 1157.62, 1172.61, 1175.64, 1175.64, 1179.62, 1184.60, 1186.62, 1191.59, 1194.59, 1197.62, 1206.56, 1207.57, 1209.59, 1223.72, 1237.61, 1255.62, 1255.62, 1277.61, 1285.71, 1307.69, 1323.66, 1398.70, 1430.69, 1442.71, 1443.75, 1457.72, 1459.75, 1462.76, 1465.73, 1473.72, 1475.75, 1481.73, 1491.74, 1497.72, 1581.90, 2230.24, 2638.38, 2682.40, 2698.42
17. gi|41322904 Mass: 519466 Score: 48 Expect: 1.7 Queries matched: 40
plectin 1 isoform 1 [Mus musculus]

作者: misswu61 时间: 2014-3-27 23:21

Observed Mr(expt) Mr(calc) Delta Start End Miss Ions Peptide
744.39 743.38 743.39 -0.01 1376 - 1381 0 --- R.LAEQQR.A
766.38 765.37 765.39 -0.02 1806 - 1812 1 --- R.STSEKSK.Q
822.38 821.37 821.32 0.05 1370 - 1375 0 --- R.MEEEER.L
961.53 960.52 960.53 -0.01 3264 - 3272 0 --- K.VEVPVGSFK.G
989.53 988.52 988.52 0.00 1485 - 1492 0 --- R.LQLETTER.Q
990.52 989.52 989.50 0.01 1599 - 1607 0 --- R.SAEVELQSK.R
1002.57 1001.56 1001.53 0.04 2393 - 2400 1 --- R.TLEAERQR.Q
1007.54 1006.54 1006.54 -0.01 1958 - 1967 1 --- K.VSFEKAAAGK.A
1007.54 1006.54 1006.54 -0.01 1958 - 1967 1 --- K.VSFEKAAAGK.A
1018.55 1017.54 1017.50 0.04 1423 - 1430 1 --- R.MQEEVARR.E
1021.52 1020.52 1020.52 -0.00 2413 - 2420 1 --- K.LRMVEMSR.A
1023.54 1022.53 1022.54 -0.01 1189 - 1196 1 --- R.LQQRHGER.D
1029.52 1028.52 1028.54 -0.02 1813 - 1821 1 --- K.QRLEAEAGR.F
1045.51 1044.50 1044.48 0.02 1040 - 1048 1 --- K.DPARECAQR.I
1072.49 1071.48 1071.58 -0.10 1388 - 1397 0 --- R.LAEVEAALEK.Q
1073.47 1072.47 1072.51 -0.05 2000 - 2008 0 --- R.QLAAEEEQR.R
1090.50 1089.49 1089.53 -0.04 1453 - 1462 0 --- R.QSSEAEIQAK.A
1090.50 1089.49 1089.52 -0.03 2867 - 2876 0 --- R.ILSDPSDDTK.G
1157.62 1156.61 1156.61 0.00 2353 - 2362 0 --- K.AEAELLQQQK.E
1172.61 1171.61 1171.67 -0.07 191 - 199 1 --- K.EKLLLWSQR.M
1175.64 1174.63 1174.52 0.11 1746 - 1755 0 7 R.AETEQGEQQR.Q
1175.64 1174.63 1174.52 0.11 1746 - 1755 0 --- R.AETEQGEQQR.Q
1179.62 1178.62 1178.65 -0.03 2820 - 2829 1 --- K.DLIVRDHGVR.L
1186.62 1185.61 1185.64 -0.02 1776 - 1785 1 --- K.RQELEAELAK.V
1191.59 1190.58 1190.60 -0.03 1822 - 1831 1 --- R.FRELAEEAAR.L
1194.59 1193.59 1193.56 0.02 2415 - 2424 1 --- R.MVEMSRAQAR.A + Oxidation (M)
1197.62 1196.61 1196.61 -0.01 1765 - 1775 0 --- R.LQHEATAATQK.R
1209.59 1208.58 1208.63 -0.05 2624 - 2632 1 --- R.LQRLEEEHR.A
1255.62 1254.61 1254.63 -0.01 3392 - 3401 1 --- K.EKVTIYEAMR.R + Oxidation (M)
1255.62 1254.61 1254.63 -0.01 3392 - 3401 1 --- K.EKVTIYEAMR.R + Oxidation (M)
1285.71 1284.70 1284.70 -0.00 1777 - 1787 1 --- R.QELEAELAKVR.A
1307.69 1306.68 1306.72 -0.04 4029 - 4040 1 --- K.LLSAERAVTGYK.D
1323.66 1322.65 1322.72 -0.07 2983 - 2993 0 --- K.IVITVVEEHER.K
1398.70 1397.69 1397.79 -0.10 2351 - 2362 1 --- R.LKAEAELLQQQK.E
1430.69 1429.69 1429.76 -0.07 640 - 653 0 --- R.SLESLHGFVAAATK.E
1443.75 1442.74 1442.75 -0.01 3034 - 3046 1 --- R.EVAEADSVRQALR.G
1457.72 1456.72 1456.62 0.10 209 - 220 1 --- R.CDNFTTSWRDGR.L
1473.72 1472.72 1472.66 0.06 490 - 501 1 --- K.DGRHPQGEQMYR.R
1481.73 1480.72 1480.72 0.01 4224 - 4236 1 --- R.SGRQYDIDDAITK.N
1497.72 1496.71 1496.61 0.11 662 - 673 0 --- K.EEEEVGFDWSDR.N
No match to: 728.15, 842.51, 870.54, 877.04, 980.58, 980.58, 1005.52, 1006.51, 1019.54, 1051.51, 1112.48, 1184.60, 1206.56, 1207.57, 1223.72, 1237.61, 1277.61, 1442.71, 1459.75, 1462.76, 1465.73, 1475.75, 1491.74, 1581.90, 2230.24, 2638.38, 2682.40, 2698.42
18. gi|41322939 Mass: 517809 Score: 48 Expect: 1.7 Queries matched: 40
plectin 1 isoform 10 [Mus musculus]

作者: misswu61 时间: 2014-3-27 23:21

Observed Mr(expt) Mr(calc) Delta Start End Miss Ions Peptide
744.39 743.38 743.39 -0.01 1354 - 1359 0 --- R.LAEQQR.A
766.38 765.37 765.39 -0.02 1784 - 1790 1 --- R.STSEKSK.Q
822.38 821.37 821.32 0.05 1348 - 1353 0 --- R.MEEEER.L
961.53 960.52 960.53 -0.01 3242 - 3250 0 --- K.VEVPVGSFK.G
989.53 988.52 988.52 0.00 1463 - 1470 0 --- R.LQLETTER.Q
990.52 989.52 989.50 0.01 1577 - 1585 0 --- R.SAEVELQSK.R
1002.57 1001.56 1001.53 0.04 2371 - 2378 1 --- R.TLEAERQR.Q
1007.54 1006.54 1006.54 -0.01 1936 - 1945 1 --- K.VSFEKAAAGK.A
1007.54 1006.54 1006.54 -0.01 1936 - 1945 1 --- K.VSFEKAAAGK.A
1018.55 1017.54 1017.50 0.04 1401 - 1408 1 --- R.MQEEVARR.E
1021.52 1020.52 1020.52 -0.00 2391 - 2398 1 --- K.LRMVEMSR.A
1023.54 1022.53 1022.54 -0.01 1167 - 1174 1 --- R.LQQRHGER.D
1029.52 1028.52 1028.54 -0.02 1791 - 1799 1 --- K.QRLEAEAGR.F
1045.51 1044.50 1044.48 0.02 1018 - 1026 1 --- K.DPARECAQR.I
1072.49 1071.48 1071.58 -0.10 1366 - 1375 0 --- R.LAEVEAALEK.Q
1073.47 1072.47 1072.51 -0.05 1978 - 1986 0 --- R.QLAAEEEQR.R
1090.50 1089.49 1089.53 -0.04 1431 - 1440 0 --- R.QSSEAEIQAK.A
1090.50 1089.49 1089.52 -0.03 2845 - 2854 0 --- R.ILSDPSDDTK.G
1157.62 1156.61 1156.61 0.00 2331 - 2340 0 --- K.AEAELLQQQK.E
1172.61 1171.61 1171.67 -0.07 169 - 177 1 --- K.EKLLLWSQR.M
1175.64 1174.63 1174.52 0.11 1724 - 1733 0 7 R.AETEQGEQQR.Q
1175.64 1174.63 1174.52 0.11 1724 - 1733 0 --- R.AETEQGEQQR.Q
1179.62 1178.62 1178.65 -0.03 2798 - 2807 1 --- K.DLIVRDHGVR.L
1186.62 1185.61 1185.64 -0.02 1754 - 1763 1 --- K.RQELEAELAK.V
1191.59 1190.58 1190.60 -0.03 1800 - 1809 1 --- R.FRELAEEAAR.L
1194.59 1193.59 1193.56 0.02 2393 - 2402 1 --- R.MVEMSRAQAR.A + Oxidation (M)
1197.62 1196.61 1196.61 -0.01 1743 - 1753 0 --- R.LQHEATAATQK.R
1209.59 1208.58 1208.63 -0.05 2602 - 2610 1 --- R.LQRLEEEHR.A
1255.62 1254.61 1254.63 -0.01 3370 - 3379 1 --- K.EKVTIYEAMR.R + Oxidation (M)
1255.62 1254.61 1254.63 -0.01 3370 - 3379 1 --- K.EKVTIYEAMR.R + Oxidation (M)
1285.71 1284.70 1284.70 -0.00 1755 - 1765 1 --- R.QELEAELAKVR.A
1307.69 1306.68 1306.72 -0.04 4007 - 4018 1 --- K.LLSAERAVTGYK.D
1323.66 1322.65 1322.72 -0.07 2961 - 2971 0 --- K.IVITVVEEHER.K
1398.70 1397.69 1397.79 -0.10 2329 - 2340 1 --- R.LKAEAELLQQQK.E
1430.69 1429.69 1429.76 -0.07 618 - 631 0 --- R.SLESLHGFVAAATK.E
1443.75 1442.74 1442.75 -0.01 3012 - 3024 1 --- R.EVAEADSVRQALR.G
1457.72 1456.72 1456.62 0.10 187 - 198 1 --- R.CDNFTTSWRDGR.L
1473.72 1472.72 1472.66 0.06 468 - 479 1 --- K.DGRHPQGEQMYR.R
1481.73 1480.72 1480.72 0.01 4202 - 4214 1 --- R.SGRQYDIDDAITK.N
1497.72 1496.71 1496.61 0.11 640 - 651 0 --- K.EEEEVGFDWSDR.N
No match to: 728.15, 842.51, 870.54, 877.04, 980.58, 980.58, 1005.52, 1006.51, 1019.54, 1051.51, 1112.48, 1184.60, 1206.56, 1207.57, 1223.72, 1237.61, 1277.61, 1442.71, 1459.75, 1462.76, 1465.73, 1475.75, 1491.74, 1581.90, 2230.24, 2638.38, 2682.40, 2698.42
19. gi|94371894 Mass: 67614 Score: 46 Expect: 2.9 Queries matched: 11
PREDICTED: similar to zinc finger protein 618 [Mus musculus]
Observed Mr(expt) Mr(calc) Delta Start End Miss Ions Peptide
744.39 743.38 743.44 -0.06 307 - 312 1 --- K.RTLQAR.S
1051.51 1050.50 1050.52 -0.02 2 - 10 1 --- M.AAENTFSRR.V
1112.48 1111.48 1111.58 -0.10 602 - 610 1 --- K.LMFLKSNML.- + Oxidation (M)
1186.62 1185.61 1185.66 -0.04 440 - 449 0 --- K.LCHLFLEALK.E
1206.56 1205.56 1205.63 -0.07 450 - 459 1 --- K.ENFKVHPAHK.V
1277.61 1276.60 1276.71 -0.11 234 - 245 1 --- K.SYVLGVKGADIR.D
1285.71 1284.70 1284.68 0.02 181 - 190 1 --- K.HLPRMYNQVK.V
1457.72 1456.72 1456.72 -0.00 277 - 290 1 --- R.VSTSAFSKAGMCLR.C
1465.73 1464.72 1464.73 -0.01 132 - 146 0 --- R.VMCADLGALSVVSGK.E + Oxidation (M)
1473.72 1472.72 1472.71 0.01 277 - 290 1 --- R.VSTSAFSKAGMCLR.C + Oxidation (M)
1497.72 1496.71 1496.65 0.06 511 - 524 0 --- R.SATGEHPAAQEEDR.L
No match to: 728.15, 766.38, 822.38, 842.51, 870.54, 877.04, 961.53, 980.58, 980.58, 989.53, 990.52, 1002.57, 1005.52, 1006.51, 1007.54, 1007.54, 1018.55, 1019.54, 1021.52, 1023.54, 1029.52, 1045.51, 1072.49, 1073.47, 1090.50, 1090.50, 1157.62, 1172.61, 1175.64, 1175.64, 1179.62, 1184.60, 1191.59, 1194.59, 1197.62, 1207.57, 1209.59, 1223.72, 1237.61, 1255.62, 1255.62, 1307.69, 1323.66, 1398.70, 1430.69, 1442.71, 1443.75, 1459.75, 1462.76, 1475.75, 1481.73, 1491.74, 1581.90, 2230.24, 2638.38, 2682.40, 2698.42
20. gi|74152994 Mass: 39674 Score: 46 Expect: 2.9 Queries matched: 7
unnamed protein product [Mus musculus]

作者: misswu61 时间: 2014-3-27 23:21

Observed Mr(expt) Mr(calc) Delta Start End Miss Ions Peptide
980.58 979.58 979.48 0.10 2 - 9 0 27 M.NIMDFNVK.K
980.58 979.58 979.48 0.10 2 - 9 0 --- M.NIMDFNVK.K
1002.57 1001.56 1001.55 0.01 170 - 178 1 --- R.LDLDAAKTR.L
1255.62 1254.61 1254.61 0.00 1 - 10 1 --- -.MNIMDFNVKK.L + Oxidation (M)
1255.62 1254.61 1254.61 0.00 1 - 10 1 --- -.MNIMDFNVKK.L + Oxidation (M)
1398.70 1397.69 1397.72 -0.03 149 - 160 1 --- R.NFIEGDYKTIAK.E
2682.40 2681.40 2681.41 -0.01 134 - 156 1 --- R.ELIQTSALNFLTPLRNFIEGDYK.T
No match to: 728.15, 744.39, 766.38, 822.38, 842.51, 870.54, 877.04, 961.53, 989.53, 990.52, 1005.52, 1006.51, 1007.54, 1007.54, 1018.55, 1019.54, 1021.52, 1023.54, 1029.52, 1045.51, 1051.51, 1072.49, 1073.47, 1090.50, 1090.50, 1112.48, 1157.62, 1172.61, 1175.64, 1175.64, 1179.62, 1184.60, 1186.62, 1191.59, 1194.59, 1197.62, 1206.56, 1207.57, 1209.59, 1223.72, 1237.61, 1277.61, 1285.71, 1307.69, 1323.66, 1430.69, 1442.71, 1443.75, 1457.72, 1459.75, 1462.76, 1465.73, 1473.72, 1475.75, 1481.73, 1491.74, 1497.72, 1581.90, 2230.24, 2638.38, 2698.42
Search Parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Variable modifications : Oxidation (M)
Mass values : Monoisotopic
Protein Mass : Unrestricted
Peptide Mass Tolerance : ± 100 ppm
Fragment Mass Tolerance: ± 0.8 Da
Max Missed Cleavages : 1
Instrument type : MALDI-TOF-TOF
Query1 (728.15,1+) : <no title>
Query2 (744.39,1+) : <no title>
Query3 (766.38,1+) : <no title>
Query4 (822.38,1+) : <no title>
Query5 (842.51,1+) : <no title>
Query6 (870.54,1+) : <no title>
Query7 (877.04,1+) : <no title>
Query8 (961.53,1+) : <no title>
Query9 (980.58,1+) : Label: G2, Spot_Id: 215653, Peak_List_Id: 101701, MSMS Job_Run_Id: 15912, Comment:
Query10 (980.58,1+) : <no title>
Query11 (989.53,1+) : <no title>
Query12 (990.52,1+) : <no title>
Query13 (1002.57,1+) : <no title>
Query14 (1005.52,1+) : <no title>
Query15 (1006.51,1+) : <no title>
Query16 (1007.54,1+) : Label: G2, Spot_Id: 215653, Peak_List_Id: 101697, MSMS Job_Run_Id: 15912, Comment:
Query17 (1007.54,1+) : <no title>
Query18 (1018.55,1+) : <no title>
Query19 (1019.54,1+) : <no title>
Query20 (1021.52,1+) : <no title>
Query21 (1023.54,1+) : <no title>
Query22 (1029.52,1+) : <no title>
Query23 (1045.51,1+) : <no title>
Query24 (1051.51,1+) : <no title>
Query25 (1072.49,1+) : <no title>
Query26 (1073.47,1+) : <no title>
Query27 (1090.50,1+) : Label: G2, Spot_Id: 215653, Peak_List_Id: 101699, MSMS Job_Run_Id: 15912, Comment:
Query28 (1090.50,1+) : <no title>
Query29 (1112.48,1+) : <no title>
Query30 (1157.62,1+) : <no title>
Query31 (1172.61,1+) : <no title>
Query32 (1175.64,1+) : Label: G2, Spot_Id: 215653, Peak_List_Id: 101700, MSMS Job_Run_Id: 15912, Comment:
Query33 (1175.64,1+) : <no title>
Query34 (1179.62,1+) : <no title>
Query35 (1184.60,1+) : <no title>
Query36 (1186.62,1+) : <no title>
Query37 (1191.59,1+) : <no title>
Query38 (1194.59,1+) : <no title>
Query39 (1197.62,1+) : <no title>
Query40 (1206.56,1+) : <no title>
Query41 (1207.57,1+) : <no title>
Query42 (1209.59,1+) : <no title>
Query43 (1223.72,1+) : <no title>
Query44 (1237.61,1+) : <no title>
Query45 (1255.62,1+) : Label: G2, Spot_Id: 215653, Peak_List_Id: 101698, MSMS Job_Run_Id: 15912, Comment:
Query46 (1255.62,1+) : <no title>
Query47 (1277.61,1+) : <no title>
Query48 (1285.71,1+) : <no title>
Query49 (1307.69,1+) : <no title>
Query50 (1323.66,1+) : <no title>
Query51 (1398.70,1+) : <no title>

作者: misswu61 时间: 2014-3-27 23:22

Query52 (1430.69,1+) : <no title>
Query53 (1442.71,1+) : <no title>
Query54 (1443.75,1+) : <no title>
Query55 (1457.72,1+) : <no title>
Query56 (1459.75,1+) : <no title>
Query57 (1462.76,1+) : <no title>
Query58 (1465.73,1+) : <no title>
Query59 (1473.72,1+) : <no title>
Query60 (1475.75,1+) : <no title>
Query61 (1481.73,1+) : <no title>
Query62 (1491.74,1+) : <no title>
Query63 (1497.72,1+) : <no title>
Query64 (1581.90,1+) : <no title>
Query65 (2230.24,1+) : <no title>
Query66 (2638.38,1+) : <no title>
Query67 (2682.40,1+) : <no title>
Query68 (2698.42,1+) : <no title>

作者: IAM007 时间: 2014-3-27 23:22

Protein scores greater than 63 are significant (p<0.05).
首先，分值大于63分的，认为是有意义的。
似乎好像同一个蛋白呢，很好的结果呢
再就是匹配上的不重复的肽段，当然是越多越好啊。
蛮好的阿。
呜呜，哪里鉴定的阿？
丰度怎么样啊？

作者: misswu61 时间: 2014-3-27 23:22

在上海复旦大学鉴定的，我们在那打了好多点，结果都不错。
当然，最大的可能性是晶体蛋白，那其他的蛋白呢？他给了我们20种蛋白，指的是该蛋白点出了晶体蛋白外包含了其他的蛋白，还是指根据荷质比这种蛋白最大的可能是晶体蛋白？因为在后面分值中感觉有些蛋白是较有意义的。

作者: misswu61 时间: 2014-3-27 23:23

我这次每块胶跑了一个重复，胶还算比较漂亮，但是比较差点时重复性就不好了，不知道怎么会这样呢？我共找到了重复性比较好的5个差异点，位置比较集中，感觉是同一类型的蛋白的不同亚型，最后的结果也证实了我的想法。虽然这种蛋白与我的实验的关系曾见报道，也就是3-4篇文献，均是从mRNA水平，可我这次又通过蛋白组的方法找到这种蛋白，还有意义吗？对了，我的干扰因素与这种蛋白的关系还没见报道。此外，我觉得奇怪的是，这次我找到的却只有晶体蛋白？有哪些原因可以解释呢？期盼着各位高手的指点！谢谢！

作者: douding66 时间: 2014-3-27 23:23

虽然这种蛋白与我的实验的关系曾见报道，也就是3-4篇文献，均是从mRNA水平，可我这次又通过蛋白组的方法找到这种蛋白，还有意义吗？对了，我的干扰因素与这种蛋白的关系还没见报道。
我怎么感觉上面这句话矛盾阿？

作者: douding66 时间: 2014-3-27 23:23

后面的分值低于63分的尽管可能与你的实验很是相关或者能很好的解释也不能考虑。
“我觉得奇怪的是，这次我找到的却只有晶体蛋白？有哪些原因可以解释呢“这说明你切取的点没有混有其他蛋白，该部分胶上蛋白分离较为成功。另外这种差异还是需要western 的验证或是其他方法的验证，尽管你认为重复性比较好，也可能是假阳性结果。试验就是这么打击人

作者: summerxx 时间: 2014-3-27 23:24

结果很好
复旦遗传所做的吗？怎么收费？

欢迎光临分析测试百科 (http://bbs.antpedia.com/)