同时做的还有另两个抗体,结果基本都出来了.........................Do you did the
same procedure as that antibody? If the answer is "yes", maybe your antibody was
not good, go back to check with that company or ask them send a specialist to do it
for you.作者: ns5fan 时间: 2014-6-18 18:15
yes, absolutely the same procedure.
The problem is, the first time I applied the antibody, it worked well at 1:500.
So now I'm bewildered, don't know which step was wrong.作者: ns5fan 时间: 2014-6-18 18:16
Oh I forgot to say "the first time" I borrowed an aliquot from other.
If the answer is "yes", maybe your antibody was
not good, go back to check with that company or ask them send a specialist to do it
for you.
Could that be true? Asking them send a specialist to do it for me? Then how much should I pay……作者: 雪山飞鹿 时间: 2014-6-18 18:16
Your last experiment antibody was borrowed from other people, so did these two antibodiesw came from same company, if they did, did they has same lot number. Also increase your Concentration also is a good idea.作者: XYZQ 时间: 2014-6-18 18:17