A second issue concerns the use of DAPI to monitor apoptosis; the standard method to do this is TUNEL staining. Wouldn't that have been a better method?
我的文章,审稿人给的意见,我做的是人晶体上皮细胞,建议我用TUNEL staining,而TUNEL法检测细胞凋亡不但操作步骤麻烦,而且其结果特异性也不强。另外,TUNEL法也无法区分凋亡与坏死。这种方法现在国外的文献中基本见不到了。建议不要采用此方法了。
我该肿么办?作者: finger 时间: 2015-6-10 15:56