本人通过实验已经验证。
1.物种：rattus
2.基因名称：GAP-43
3.模板：cDNA
4.PCR类型及目的：RT-PCR
5.forward primer(5’-3’): ACAAGGAAAAAGCTCAAAG
reverse primer(5’-3’)：GACGGGGAGTTATACGTGG
6.产物长度（bp）:292 bp
7.引物浓度：20pmol/L
8.退火温度：58℃
9提交者ID：rucy1976
10参考文献:Leung MS, Wong CC. Expressions of putative neurotransmitters and neuronal growth related genes in Merkel cell-neurite complexes of the rats. Life Science, 2000; 66(16):1481-9作者: 葡萄籽 时间: 2011-8-21 13:29
真菌ITS序列引物
Internal transcribed spacer (ITS) region primers
The ITS region is now perhaps the most widely sequenced DNA region in fungi. It has typically been most useful for molecular systematics at the species level, and even within species (e.g., to identify geographic races). Because of its higher degree of variation than other genic regions of rDNA (SSU and LSU), variation among individual rDNA repeats can sometimes be observed within both the ITS and IGS regions. In addition to the standard ITS1+ITS4 primers used by most labs, everal taxon-specific primers have been described that allow selective amplification of fungal sequences (e.g., see Gardes & Bruns 1993 paper describing amplification of basidiomycete ITS sequences from mycorrhiza samples).
primer name sequence (5'->3') comments reference
ITS1 TCCGTAGGTGAACCTGCGG White et al, 1990
ITS2 GCTGCGTTCTTCATCGATGC (is similar to 5.8S below) White et al, 1990
ITS3 GCATCGATGAAGAACGCAGC (is similar to 5.8SR below) White et al, 1990
ITS4 TCCTCCGCTTATTGATATGC White et al, 1990
ITS5 GGAAGTAAAAGTCGTAACAAGG (is similar to SR6R) White et al, 1990
ITS1-F CTTGGTCATTTAGAGGAAGTAA Gardes & Bruns, 1993
ITS4-B CAGGAGACTTGTACACGGTCCAG Gardes & Bruns, 1993
5.8S CGCTGCGTTCTTCATCG Vilgalys lab
5.8SR TCGATGAAGAACGCAGCG Vilgalys lab
SR6R AAGWAAAAGTCGTAACAAGG Vilgalys lab 作者: 葡萄籽 时间: 2011-8-21 14:29
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