Staining Procedure
1. Prepare 10-50 µM PI solution with PBS or an appropriate buffer.a)
2. Add PI solution with 1/10 of the volume of cell culture medium to the cell culture.b)
3. Incubate the cell at 37 ºC for 10-20 min.
4. Wash cells twice with PBS or an appropriate buffer.
5. Observe the cells using a fluorescence microscope with 535 nm excitation and 615 nm emission filters.
a) Since PI may be carcinogenic, extreme care is necessary during handling.
b) Or you may replace the culture medium with 1/10 concentration of EB buffer solution.作者: SO2 时间: 2015-12-11 11:16
