去Pubmed找到大鼠11β-HSD1序列,引物中间的大小(含引物)就是你的产物大小。
real time产物一般在200以内。
你也可以自己设计引物
非常感谢你的指点,但“引物中间的大小(含引物)就是你的产物大小”这句话我不明白,我选了第一个大鼠的序列,粘贴了后面的描述文字,这里面那个是指产物大小啊?
NCBI Reference Sequence: NM_017080.2
Rattus norvegicus hydroxysteroid 11-beta dehydrogenase 1 (Hsd11b1), mRNA
CommentFeaturesSequence
LOCUS NM_017080 1585 bp mRNA linear ROD 12-APR-2009
DEFINITION Rattus norvegicus hydroxysteroid 11-beta dehydrogenase 1 (Hsd11b1),
mRNA.
ACCESSION NM_017080
VERSION NM_017080.2 GI:78214364
KEYWORDS .
SOURCE Rattus norvegicus (Norway rat)
ORGANISM Rattus norvegicus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
REFERENCE 1 (bases 1 to 1585)
AUTHORS Liu,Y., Mladinov,D., Pietrusz,J.L., Usa,K. and Liang,M.
TITLE Glucocorticoid response elements and 11 beta-hydroxysteroid
dehydrogenases in the regulation of endothelial nitric oxide
synthase expression
JOURNAL Cardiovasc. Res. 81 (1), 140-147 (2009)
PUBMED 18716005
REMARK GeneRIF: Endogenous 11 beta-HSD1 and 11 beta-HSD2 play significant
and distinct roles in modulating the effect of glucocorticoids on
eNOS expression.
REFERENCE 2 (bases 1 to 1585)
AUTHORS Liu,Y., Singh,R.J., Usa,K., Netzel,B.C. and Liang,M.
TITLE Renal medullary 11 beta-hydroxysteroid dehydrogenase type 1 in Dahl
salt-sensitive hypertension
JOURNAL Physiol. Genomics 36 (1), 52-58 (2008)
PUBMED 18826995
REMARK GeneRIF: suppression of 11 beta-hydroxysteroid dehydrogenase type 1
expression in the renal medulla attenuates salt-induced
hypertension in Dahl salt-sensitive rats.
REFERENCE 3 (bases 1 to 1585)
AUTHORS Czegle,I., Margittai,E., Senesi,S., Benedetti,A. and Banhegyi,G.
TITLE Different expression and distribution of 11beta-hydroxysteroid
dehydrogenase type 1 in obese and lean animal models of type 2
diabetes
JOURNAL Acta Physiol Hung 95 (4), 419-424 (2008)
PUBMED 19009916
REMARK GeneRIF: prereceptorial glucocorticoid activation is different in
the liver and adipose tissue of the two diabetes rat models might
be responsible for the obese and lean phenotypes in type 2 diabetes
REFERENCE 4 (bases 1 to 1585)
AUTHORS McCormick,K.L., Wang,X. and Mick,G.J.
TITLE Modification of microsomal 11beta-HSD1 activity by cytosolic
compounds: glutathione and hexose phosphoesters
JOURNAL J. Steroid Biochem. Mol. Biol. 111 (1-2), 18-23 (2008)
PUBMED 18550363
REMARK GeneRIF: Modification of microsomal Hsd11b1 activity by cytosolic
compounds: glutathione and hexose phosphoesters is reported.
REFERENCE 5 (bases 1 to 1585)作者: bring 时间: 2016-4-8 15:08
AUTHORS Paulsen,S.K., Nielsen,M.P., Richelsen,B., Bruun,J.M., Flyvbjerg,A.
and Pedersen,S.B.
TITLE Upregulation of adipose 11-beta-hydroxysteroid dehydrogenase type 1
expression in ovariectomized rats is due to obesity rather than
lack of estrogen
JOURNAL Obesity (Silver Spring) 16 (4), 731-735 (2008)
PUBMED 18379559
REMARK GeneRIF: The upregulation of 11beta-HSD1 in ovariectomized rats was
most likely due to changes in body composition rather than lack of
estrogen
REFERENCE 6 (bases 1 to 1585)
AUTHORS Obeid,J. and White,P.C.
TITLE Tyr-179 and Lys-183 are essential for enzymatic activity of 11
beta-hydroxysteroid dehydrogenase
JOURNAL Biochem. Biophys. Res. Commun. 188 (1), 222-227 (1992)
PUBMED 1417845
REFERENCE 7 (bases 1 to 1585)
AUTHORS Moisan,M.P., Edwards,C.R. and Seckl,J.R.
TITLE Differential promoter usage by the rat 11 beta-hydroxysteroid
dehydrogenase gene
JOURNAL Mol. Endocrinol. 6 (7), 1082-1087 (1992)
PUBMED 1508221
REFERENCE 8 (bases 1 to 1585)
AUTHORS Krozowski,Z., Obeyesekere,V., Smith,R. and Mercer,W.
TITLE Tissue-specific expression of an 11 beta-hydroxysteroid
dehydrogenase with a truncated N-terminal domain. A potential
mechanism for differential intracellular localization within
mineralocorticoid target cells
JOURNAL J. Biol. Chem. 267 (4), 2569-2574 (1992)
PUBMED 1733955
REFERENCE 9 (bases 1 to 1585)
AUTHORS Agarwal,A.K., Monder,C., Eckstein,B. and White,P.C.
TITLE Cloning and expression of rat cDNA encoding corticosteroid 11
beta-dehydrogenase
JOURNAL J. Biol. Chem. 264 (32), 18939-18943 (1989)
PUBMED 2808402
REFERENCE 10 (bases 1 to 1585)
AUTHORS Monder,C., Shackleton,C.H., Bradlow,H.L., New,M.I., Stoner,E.,
Iohan,F. and Lakshmi,V.
TITLE The syndrome of apparent mineralocorticoid excess: its association
with 11 beta-dehydrogenase and 5 beta-reductase deficiency and some
consequences for corticosteroid metabolism
JOURNAL J. Clin. Endocrinol. Metab. 63 (3), 550-557 (1986)
PUBMED 3460996
COMMENT VALIDATED REFSEQ: This record has undergone validation or
preliminary review. The reference sequence was derived from
CK474613.1 and BC078865.1.
On Oct 28, 2005 this sequence version replaced gi:8393569.
Summary: catalyzes the interconversion of cortisol and cortisone;
plays a role in glucocorticoid metabolism; may regulate blood
pressure [RGD].
Publication Note: This RefSeq record includes a subset of the
publications that are available for this gene. Please see the
Entrez Gene record to access additional publications.
PRIMARY REFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP
1-179 CK474613.1 18-196
180-1585 BC078865.1 1-1406
FEATURES Location/Qualifiers
source 1..1585
/organism="Rattus norvegicus"
/mol_type="mRNA"
/db_xref="taxon:10116"
/chromosome="13"
/map="13q27"
gene 1..1585
/gene="Hsd11b1"
/gene_synonym="LRRGT00065"
/note="hydroxysteroid 11-beta dehydrogenase 1"
/db_xref="GeneID:25116"
/db_xref="RATMAP:39283"
/db_xref="RGD:2834"
CDS 340..1206
/gene="Hsd11b1"
/gene_synonym="LRRGT00065"
/EC_number="1.1.1.146"
/note="hydroxysteroid dehydrogenase, 11 beta type 1;
cortocosteroid 11-beta dehydrogenase"
/codon_start=1
/product="hydroxysteroid 11-beta dehydrogenase 1"
/protein_id="NP_058776.2"
/db_xref="GI:78214365"
/db_xref="GeneID:25116"
/db_xref="RATMAP:39283"
/db_xref="RGD:2834"
/translation="MKKYLLPVLVLCLGYYYSTNEEFRPEMLQGKKVIVTGASKGIGR
EMAYHLSKMGAHVVLTARSEEGLQKVVSRCLELGAASAHYIAGTMEDMAFAERFVVEA
GKLLGGLDMLILNHITQTTMSLFHDDIHSVRRSMEVNFLSYVVLSTAALPMLKQSNGS
IAIISSMAGKMTQPLIASYSASKFALDGFFSTIRKEHLMTKVNVSITLCVLGFIDTET
ALKETSGIILSQAAPKEECALEIIKGTVLRKDEVYYDKSSWTPLLLGNPGRRIMEFLS
LRSYNRDLFVSN"作者: bring 时间: 2016-4-8 15:11