目前看来可能是比较好的方法了。
最近中南大学在BMC molecular biology 发了篇甲基化的论文,所用的方法可以说是目前做甲基化的经典,该杂志IF 在3.3左右。感兴趣可看看
cuturl('http://www.ncbi.nlm.nih.gov/pubmed/18976507?ordinalpos=5&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum')
BMC Mol Biol. 2008 Nov 3;9(1):99. [Epub ahead of print]
Promoter Hypermethylation-mediated Inactivation of LRRC4 in Gliomas.Zhang Z, Li D, Wu M, Xiang B, Wang L, Zhou M, Chen P, Li X, Shen S, Li G.
PMID: 18976507 [PubMed - as supplied by publisher]作者: one 时间: 2016-4-11 14:17
我做的BSP的测序图,哪位高人给指点下是怎么回事?怎么C峰是个又大又宽的峰啊?
能不能算数啊,怎么分析呢?多谢!!作者: one 时间: 2016-4-11 14:18
应该没有问题,那几个大峰是蓝色的,ATGC都不是蓝色,并没有影响你的测序结果。
你也可以咨询一下测序公司问问是怎么回事?一般来说是没有的。可能是试剂不太好,出现的杂乱信号。作者: one 时间: 2016-4-11 14:25
推荐一篇reviewer,虽然是几年前的,但很经典的。
第一篇:
Wood, A. J. & R. J. Oakey (2006) Genomic imprinting in mammals: emerging themes and established theories. PLoS Genet, 2, e147.
Abstract: The epigenetic events that occur during the development of the mammalian embryo are essential for correct gene expression and cell-lineage determination. Imprinted genes are expressed from only one parental allele due to differential epigenetic marks that are established during gametogenesis. Several theories have been proposed to explain the role that genomic imprinting has played over the course of mammalian evolution, but at present it is not clear if a single hypothesis can fully account for the diversity of roles that imprinted genes play. In this review, we discuss efforts to define the extent of imprinting in the mouse genome, and suggest that different imprinted loci may have been wrought by distinct evolutionary forces. We focus on a group of small imprinted domains, which consist of paternally expressed genes embedded within introns of multiexonic transcripts, to discuss the evolution of imprinting at these loci.作者: pore 时间: 2016-4-11 15:44