我觉得是完全可以的,理由是:
我购买gibco公司的dmem-f12培养基的使用说明书明确的写到:use of 1N NaOH or 1N HCl is recommended.
D-MEM/F-12, Gibco, 12400-024说明书(我做了简单的翻译,仅供参考):
1) measure out 5% less distilled water than desired total volume of medium, using a mixing container that is as close to the final volume as possible.
量出少于总量5%的蒸馏水
2) add powered medium to 15 to 30℃ (room temperature) water with gentle stirring. (Do not heart water).
加入干粉培养基,轻轻搅拌(不能加热)
3) rinse out inside of package to remove all traces of power.
冲洗包装袋 内壁
4) add 1.2 g of NaHCO3 per liter of medium.
加入1.2g碳酸氢钠(每升)
5) dilute to a desired volume with water. Stir until dissolved. (do not over-mix)
稀释至所需水量(轻轻搅拌,不要加过量了)
6) adjust pH of medium to 0.2-0.3 below desired final working*:use of 1N NaOH or 1N HCl is recommended.(add slowly with stirring). After pH has been adjuster, keep container closed until medium is filtered.
调节ph值(比最后要求的ph低0.2-0.3,因为过滤以后会上升0.1-0.3*pH units will usually rise 0.1-0.3 upon filtration),用1N氢氧化钠或者1N盐酸调节,)调好后封装避免灰尘。
8) sterilize immediately by membrane filtration. (positive pressure recommended).
立即用滤膜正压过滤
*pH units will usually rise 0.1-0.3 upon filtration