【讨论帖】一种细胞检测MTT法改良试剂测评 - 经验共享

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标题:【讨论帖】一种细胞检测MTT法改良试剂测评

66+77[使用道具]
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发表于 2012-10-19 12:37 资料个人空间短消息加为好友

小中大

我再贴
5.
AZUHIRO WASHIO, MOTOI AOE, SHINICHI TOYOOKA, HIROYUKI MUSHIAKE, KAZUNORI TSUKUDA and NOBUYOSHI SHIMIZUThe effect of epidermal growth factor receptor antisense morpholino oligomer on non-small cell lung cancer cell lineONCOLOGY REPORTS 10: 1967-1971, 2003
cuturl('http://virology.med.uoc.gr/OR/2003/volume10/number6/1967.pdf')
6.
YOSHIMASA INOUE, MASASHI TOMISAWA, HITOSHI YAMAZAKI, YOSHIYUKI ABE,HIROSHI SUEMIZU, HIDEO TSUKAMOTO, YASUSHI TOMII, MASAFUMI KAWAMURA, HIROSHI KIJIMA,HIROYUKI HATANAKA, YO***O UEYAMA, MASATO NAKAMURA and KOICHI KOBAYA***he modifier subunit of glutamate cysteine ligase (GCLM) is a molecular target for amelioration of cisplatin resistance in lung cancerINTERNATIONAL JOURNAL OF ONCOLOGY 23: 1333-1339, 2003
cuturl('http://virology.med.uoc.gr/IJO/2003/volume23/number5/1333.pdf')
7.
Kazuhiro Takahashi, Kazuhito Totsune , Tomomi Kitamuro, Masahiko Sone, Osamu Murakami and Shigeki ShibaharaThree vasoactive peptides, endothelin-1, adrenomedullin and urotensin-II, in human tumour cell lines of different origin: expression and effects on proliferationClinical Science, 103(48), 35S-38S (2002).
cuturl('http://www.clinsci.org/cs/103/035S/103035S.pdf')
^ ^

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发表于 2012-10-19 12:37 资料个人空间短消息加为好友

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又有一些新的cck-8试剂文献了：）
8
Toshiyuki Araki, Masashi Yamada, Hiroshi Ohnishi, Shin-ichiro Sano, Taichi Uetsuki and Hiroshi Hatanaka Shp-2 Specifically Regulates Several Tyrosine-Phosphorylated Proteins in Brain-Derived Neurotrophic Factor Signaling inCultured Cerebral Cortical NeuronsJ. Neurochem. 74, 659–668 (2000)..
cuturl('http://www.blackwell-synergy.com/servlet/useragent?func=synergy&synergyAction=showTOC&journalCode=jnc&volume=74&issue=2&year=2000&part=null')
9
Yoshiyuki Shibata and Takashi NakamuraSignificant Antitumoral Activity of Cationic Multilamellar Liposomes Containing Human IFN-β Gene againstHuman Renal Cell CarcinomaClinical Cancer Research Vol. 9, 1129–1135, March 2003
cuturl('http://cnc.cj.uc.pt/~PhDprog/GeneTher/4%20Nakanishi.pdf')
Yoshiaki Morita, Tetsuji Naka, Yoshinori Kawazoe, Minoru Fujimoto, Masashi Narazaki, 10
Reiko Nakagawa, Hidehiro Fukuyama, Shigekazu Nagata, and Tadamitsu KishimotoSignals transducers and activators of transcription (STAT)-induced STAT inhibitor-1 (SSI-1)/suppressor of cytokine signaling-1 (SOCS-1) suppresses tumor necrosis factor -induced cell death in fibroblastsProc. Natl. Acad. Sci. USA, 97, 5405 - 5410 (2000)
cuturl('http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=25841&action=stream&blobtype=pdf')

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发表于 2012-10-19 12:38 资料个人空间短消息加为好友

小中大

今天刚测了MTT的结果,效果又不好,能否给我提供四块板的试剂,我同时做两种细胞系和两种药物的,谢谢!
我的地址已发到你的邮箱了!谢谢!

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发表于 2012-10-19 12:39 资料个人空间短消息加为好友

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10
Satomi Onoue, Keiichi Ohshima, Kosuke Endo, Takehiko Yajima and Kazuhisa Kashimoto PACAP protects neuronal PC12 cells from the cytotoxicity of human prion protein fragment 106-126FEBS LettersVolume 522, Issue 1-3, pp. 65-70.
cuturl('http://www1.elsevier.com/febs/427/19/33/main.pdf')

11
Jun Nakamura, Shoji Asakura, Susan D. Hester, Gilbert de Murcia, Keith W. Caldecott and James A. SwenbergPrevalent Loss of Mitotic Spindle Checkpoint in Adult T-cell Leukemia Confers Resistance to Microtubule InhibitorsJ. Biol. Chem., Vol. 277, 5187-5193, 2002
cuturl('http://www.niaid.nih.gov/dir/labs/lmm/jeang/Biblio/kasaijbc2002.pdf')

12
R Fujita and H UedaProtein kinase C-mediated cell death mode switch induced by high glucoseCell death and Differentiation (2003) 00, 1-12
cuturl('http://www.ph.nagasaki-u.ac.jp/lab/neuro/doc/mol.pharmacol./pdf_file/Publications_pdf/2003CDD10_p1336.pdf')

13
Jun Nakamura, Shoji Asakura, Susan D. Hester, Gilbert de Murcia, Keith W. Caldecott and James A. SwenbergQuantitation of intracellular NAD(P)H can monitor an imbalance of DNA single strand break repair in base excision repair deficient cells in real timeNucleic Acids Research, 2003, Vol. 31, No. 17
cuturl('http://nar.oupjournals.org/cgi/reprint/31/17/e104.pdf')

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14
Toshiyuki Araki, Masashi Yamada, Hiroshi Ohnishi, Shin-ichiro Sano, Taichi Uetsuki and Hiroshi Hatanaka Shp-2 Specifically Regulates Several Tyrosine-Phosphorylated Proteins in Brain-Derived Neurotrophic Factor Signaling inCultured Cerebral Cortical NeuronsJ. Neurochem. 74, 659–668 (2000)..
cuturl('http://www.blackwell-synergy.com/servlet/useragent?func=synergy&synergyAction=showTOC&journalCode=jnc&volume=74&issue=2&year=2000&part=null')

15
Yoshiyuki Shibata and Takashi NakamuraSignificant Antitumoral Activity of Cationic Multilamellar Liposomes Containing Human IFN-β Gene againstHuman Renal Cell CarcinomaClinical Cancer Research Vol. 9, 1129–1135, March 2003
cuturl('http://cnc.cj.uc.pt/~PhDprog/GeneTher/4%20Nakanishi.pdf')
16
Yoshiaki Morita, Tetsuji Naka, Yoshinori Kawazoe, Minoru Fujimoto, Masashi Narazaki, Reiko Nakagawa, Hidehiro Fukuyama, Shigekazu Nagata, and Tadamitsu KishimotoSignals transducers and activators of transcription (STAT)-induced STAT inhibitor-1 (SSI-1)/suppressor of cytokine signaling-1 (SOCS-1) suppresses tumor necrosis factor -induced cell death in fibroblastsProc. Natl. Acad. Sci. USA, 97, 5405 - 5410 (2000)
cuturl('http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=25841&action=stream&blobtype=pdf')

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发表于 2012-10-19 12:40 资料个人空间短消息加为好友

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试用品够作几个孔？

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发表于 2012-10-19 12:41 资料个人空间短消息加为好友

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QUOTE:

原帖由 S6044 于 2012-10-19 12:40 发表 bbcodeurl('http://bbs.antpedia.com/images/common/back.gif', '%s')

试用品够作几个孔？

您好：
试用品够做一块板的。

qhyu[使用道具]
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发表于 2012-10-19 12:41 资料个人空间短消息加为好友

小中大

用CCk－8做生长曲线，是不是应该要3～5块96孔板？

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发表于 2012-10-19 12:42 资料个人空间短消息加为好友

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17.
Yuichi Hashimoto, Hong Jiang, Takako Niikura, Yuko Ito, Akari Hagiwara, Kazuo Umezawa, Yoichiro Abe, Yo***ake Murayama, and Ikuo NishimotoNeuronal Apoptosis by Apolipoprotein E4 through Low-Density Lipoprotein Receptor-Related Protein and Heterotrimeric GTPases The Journal of Neuroscience, November 15, 2000, 20(22):8401–8409.
cuturl('http://www.jneurosci.org/cgi/reprint/20/22/8401.pdf')

18.
YUICHI HASHIMOTO, TAKAKO NIIKURA, YUKO ITO, YOSHIKO KITA, KENZO TERA***A, and IKUO NISHIMOTONeurotoxic Mechanisms by Alzheimer’s Disease-Linked N141I Mutant Presenilin 2THE JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS Vol. 300, No. 3
cuturl('http://jpet.aspetjournals.org/cgi/reprint/300/3/736.pdf')

19.
Masayasu Kimura, Yoichi Mizukami, Toshiro Miura, Kiyoko Fujimoto, Sei Kobayashi and Masunori MatsuzakiOrphan G Protein-coupled Receptor, GPR41, Induces Apoptosis via a p53/Bax Pathway during Ischemic Hypoxia and ReoxygenationJ. Biol. Chem., 276(28), 26453-26460 (2001).
cuturl('http://www.jbc.org/cgi/reprint/276/28/26453')

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发表于 2012-10-19 12:42 资料个人空间短消息加为好友

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20
Issei Imoto, Zeng-Quan Yang, Atiphan Pimkhaokham, Hitoshi Tsuda, Yutaka Shimada, Masayuki Imamura, Misao Ohki, and Johji InazawaIdentification of cIAP1 As a Candidate Target Gene within an Amplicon at 11q22 in Esophageal Squamous Cell CarcinomasCANCER RESEARCH 61, 6629–6634, September 15, 2001
cuturl('http://cancerres.aacrjournals.org/cgi/reprint/61/18/6629.pdf')
21
Tetsuo Udono, Kazuhiro Takahashi, Masaharu Nakayama, Ayako Yoshinoya, Kazuhito Totsune, Osamu Murakami, Yusuf K. Durlu, Makoto Tamai, and Shigeki ShibaharaInduction of Adrenomedullin by Hypoxia in Cultured Retinal Pigment Epithelial CellsIOVS, April 2001, Vol. 42, No. 5
cuturl('http://www.iovs.org/cgi/reprint/42/5/1080.pdf')
22
Michael L. Spencer, Haipeng Shao, and Douglas A. AndresInduction of Neurite Extension and Survival in Pheochromocytoma Cells by the Rit GTPaseJ. Biol. Chem., 277(23), 20160-20168 (2002).
cuturl('http://www.jbc.org/cgi/reprint/277/23/20160?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&andorexactfulltext=and&searchid=1081494519943_20&stored_search=&FIRSTINDEX=0&sortspec=relevance&volume=277&firstpage=20160&resourcetype=1')
23
Jeng-Long Hsieh, Chao-Liang Wu, Che-Hsin Lee, and Ai-Li ShiauHepatitis B Virus X Protein Sensitizes Hepatocellular Carcinoma Cells to Cytolysis Induced by E1B-deleted Adenovirus through the Disruption of p53 FunctionClinical Cancer Research Vol. 9, 338–345, January 2003
cuturl('http://clincancerres.aacrjournals.org/cgi/reprint/9/1/338.pdf')
24
Yasushi Tomii, Junichiro Kamochi, Hitoshi Yamazaki, Nobuko Sawa, Tetsuji Tokunaga, Yasuyuki Ohnishi, Hiroshi Kijima, Yo***o Ueyama, Norikazu Tamaoki and Masato Nakamura Human Thrombospondin 2 Inhibits Proliferation of Microvascular Endothelial Cells Int. J. Oncol., 20, 339-342 (2002).
cuturl('http://virology.med.uoc.gr/IJO/2002/volume20/number2/339-342.pdf')
不知道这些论文对你们有没有用

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