小中大
1.ECV304已经不被认可为内皮细胞了,来源于膀胱癌上皮,在板块内可搜索到;所以,如果想做内皮的实验,该株充其量可以用来做预试;
2.
CRL-1730
Yes, the cells did form colonies in semisolid medium.
No, the cells were not tumorigenic in immunosuppressed mice
This is a hypodiploid human cell line. The modal chromosome number was 45 occurring in 72% of cells counted. The rate of polyploid cells was 15.8%. All cells had monosomic N13 and the subclone with additional monosomic N15 predominates. Other coexisting subclones include those with 46,XX,-11,-13,i(11p),i(11q) and 46,XX,+11,-13 karyotypes. Both X chromosomes appear normal.
亚二倍体细胞,但是没有致瘤性,所以不算肿瘤细胞。
3.CRL-2480
A frozen ampule at population doubling 20 was received at the ATCC in June, 1999.
The cells will senesce at about population doubling level (PDL) 45. Check product sheet to see the PDL of the current distribution stock.
二倍体细胞,算正常细胞,可以传代45代;
关键是你要用来做什么实验?
个人认为CRL-2480好一些,可以当脐静脉内皮细胞用。不过,黄花菜,你也可以自己做原代呀?
有战友买过后面两株细胞的吗?作个评论怎么样?