小中大我的peg-丁醛是在贵公司买的,修饰率还不错,但会有多点修饰,我想请教一下,您说的(3)PEG分子量标准,PEG染色,SDS-PAGE,在哪里能买到?
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Staining Procedure
After electrophoresis the gel was soaked in a 5% glu-
taraldehyde (Merck) solution for 15 min at room tem-
perature for fixation. Afterward the gel was stained for
PEG as follows. First, the gel was put in 20 ml of perchlo-
ric acid (0.1 M) for 15 min, and then 5 ml of a 5% barium
chloride solution (Riedel de Haen) and 2 ml of a 0.1 M
iodine solution (Merck, Titrisol 9910) were added ac-
cording to the procedure described by Skoog (3).
ANALYTICAL BIOCHRMISTRY 200,244-248 (1992)
上传不了整个文件,只能将PEG染色部分摘录,另外醛基修饰产生多修饰,你可以将修饰缓冲液pH降低