小中大请教几个问题：
关于嗜硫，proteinA和proteinG不是太明白他们的区别：
1.嗜硫(Thiophilic Resin)和 protein A resin的区别：
在clontech公司网站上这样介绍嗜硫树脂：
Both Thiophilic-Uniflow and -Superflow Resins utilize thiophilic adsorption chromatography. With this technique, protein adsorbs to a sulfone thioether ligand. The adsorption of different proteins can be promoted by adding different salts to the mixture. Varying the concentration of the loading salt can affect the adsorption affinities of IgG, IgM, IgA, Fab and Fc fragments, and C3 and C4 complement factors (1).
Purification with Thiophilic Resins offers several advantages over purification with Protein A, the conventional method used for immunoglobulin purification:
Purification at neutral pH
High linear flow rates
High capacity (15–20 mg Ab/ml resin)
Broader selectivity (IgY, IgM, IgE, scAb)
Reusability
Higher stability of the purified product
好像嗜硫树脂的优点非常多，但是在实际应用中，好像大部分人还是倾向于protein A resin。我没有实际应用过这两种树脂，想问一下实际应用中这两种树脂的差别？
2.proteinA和proteinG的区别：
我知道在免疫沉淀实验时候同时用proteinA和proteinG效果会比较好，但是他们的区别是什么呢？何时用proteinA比较好，何时用proteinG比较好呢？
3.据我了解，proteinA有普通蛋白的也有重组蛋白的，重组蛋白会删掉一些非特异结合区域。想问一下在实际应用中你们用那种比较多？如果我想开发商品化的proteinA resin，从使用者角度对我有什么建议？
不好意思一下问了这么多问题，有的可能比较初级。
先谢谢了！
