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Benzonase
<enzyme> Product of genetic engineering which hydrolyzes both DNA and RNA; from serratia marcescens(粘质沙雷氏菌);both Dnase and Rnase activity.
Synonym: benzon nuclease, ben endonuclease
Benzonase® - The smart solution for DNA removal
For the first time, an effective biochemical method is available for removing DNA and RNA from laboratory and industrial scale bioprocesses. It is called Benzonase® endonuclease.
Benzonase® is a unique, genetically engineered endonuclease offering a variety of advantages over existing methods of nucleic acid removal.
Benzonase® endonuclease has proven its value in the laboratory for well over ten years and is successfully applied in the pharmaceutical and biotechnological industry in the downstream processing of biopharmaceutical actives e.g. monoclonal antibodies, virus vaccines, gene therapy and recombinant proteins from E.coli.
Benzonase® is applied whenever the presence of nucleic acids might cause interference i.e.
• Purification and characterisation of proteins, and peptides
• Purification of antibodies and viruses for therapeutic use
• Safety of biopharmaceuticals
FDA guidelines for the manufacture of recombinant biological entities for therapeutic use demand that nucleic acid contamination should be limited to 10 pg per dose. Benzonase® - alone or in combination with other steps - offers a powerful and well-accepted tool to achieve regulatory requirements.
Benzonase® is covered by several patents, i.e. US Patent No.5,173,418, EP Patent No.0229866 B1
