Figure 1. (a) Experimental scheme of the SEI experiment. The pulse
sequence incorporates a Gaussian-shaped pulse of angle R e 90°, followed
by a modified WATERGATE sequence for residual water signal suppression.
The rSnob profile9 is used for selective refocusing of downfield shifted
protein resonances in the spin-echo part of the experiment (the 2 ms long
rSnob is placed in the middle of the Δ ) 4 ms delay). The Gaussian pulse
has the duration of 1 ms and R of 77°. Selective pulses are applied at 10
ppm. Gradient strengths are 22% and 20% for G1 and G2, respectively; δ
was set to 100 μs, the gradient recovery delay to 100 μs, and the acquisition
time t1 to 300 ms, giving the total scan time Tscan ～ 300 ms. (b) (c) 1D 1H
NMR spectra of the 35 μM Mdm2-p53 complex; twoH signals of Trp
of p53 are visible (for the NMR properties of the complex see Figure S2).
Both spectra were acquired in 2.5 min using (b) the SEI pulse sequence
and (c) a 90° hard pulse followed by the WATERGATE-W3 sequence.
我在看JACS上一篇关于
核磁共振NMR筛选化合物活性的文章，里面提及一些内容，哪位高人能否对这图的说明做个解释翻译吗？尤其是（a），我看了半天都没弄懂啥意思。是不是一些参数的设置啊？谢谢
