细胞生物学

　　利用PHOTOSHOP将两种荧光图象重叠，从而达到激光共聚焦效果，可以节省不少 操作步骤如下：

　　Photoshop Procedure to Colorize Immuno Images

　　Open Photoshop

　　Open both images (Cy3-red and Hoechest33258-blue)

　　1. Choose one image

　　Image—Mode—RGB Color

　　Image—Adjust—Brightness/Contrast.

　　Then Save As:

　　2. Choose other image

　　Image—Mode—RGB Color

　　Image—Adjust—Brightness/Contrast.

　　Then Save As:

　　3. Select one of the images—(say the Hoechest33258-blue)

　　Go to Channels

　　Click on Red—Go to Image—Adjust—Brightness/Contrast

　　Move both sliders to zero (or -100)

　　Click on Green—Go to Image—Adjust—Brightness/Contrast

　　Move both sliders to zero (or -100)

　　Save

　　4. Select the other image (Cy3-red)

　　Go to Channels

　　Click on Green—Go to Image—Adjust—Brightness/Contrast

　　Move both sliders to zero (or -100)

　　Click on Blue—Go to Image—Adjust—Brightness/Contrast

　　Move both sliders to zero (or -100)

　　Save

　　5. Select one of the images

　　Go to Layers

　　Double click on Background—Rename: Cy3-red

　　Save

　　6. Select the other image

　　Go to Layers

　　Double click on Background—Rename: Hoechest33258-blue

　　Save

　　7. Select All

　　Copy

　　8. Go to the other image

　　Paste

　　Change from “Normal” to “Screen” on Layers pallette.

　　Save As: