小中大
介绍一根Shodex的氨基酸分析柱.(中国总代理:北京龙智达公司,北京010-63444635,email:longzida@263.net//或和SEPU.NET网站联系)
Amino acid standards are analyzed using Shodex CXpak P-421S.
This column is packed the 6um of strong cation exchanged gel, and whose dimension is 4.6mm ID and 150mm in length. The column pressure is low ( 3MPa under 0.5mL/min and 6 3deg-C), because of the 6um of gel is packed into this column.
We show the chromatogram of standard amino acids under ninhydrine reaction. 17 kinds of amino acids are separated by baseline. The total time for analysis is within 50 min till the last peak of Alginene.
Sample
1. Aspartic acid(Asp) 2. Threonine(Thr) 3. Serine(Ser) 4. Glutamic acid(Glu)
5. Proline(Pro) 6. Glycine(Gly) 7. Alanine(Ala) 8. Cysteine(Cys) 9. Valine(Val)
10. Methionine(Met) 11. Isoleucine(Ile) 12. Leucine(Leu) 13. Tyrocine(Tyr) 14. Phenylalanine(Phe)
15. Lysine(Lys) 16. NH3 17. Histidine(His) 18. Arginine(Arg)
Instrument :Sic Aminolyser 24(SYSTEM INSTRUMENTS CO.,LTD.)
Column :Shodex CXpak P-421S
Eluent :No.1 (0.0min.), No.2(1.2min.), No.3(10.0min.)
No.4(21.2min.), No.5(40min.)
No.1 Sodium citrate buffer (pH 3.3)
No.2 Sodium citrate buffer (pH 3.2)
No.3 Sodium citrate buffer (pH 4.0)
No.4 Sodium citrate buffer (pH 4.9)
No.5 Rinse Solution
Flow rate :0.5mL/min
Detect :Ninhydrine reaction (570nm, 0.35mL/min, 120 deg-C)
Column Temp.:63 deg-C
